评估用于检测自然感染和实验感染牛带绦虫囊尾蚴的牛抗体的ELISA检测方法。

Evaluation of the ELISA test for the antibody detection in cattle naturally and experimentally infected with Cysticerccus bovis.

作者信息

Monteiro L L, Pinto P S A, Dias F S

机构信息

Department of Veterinary, Viçosa Federal University, 36570-000 Viçosa, MG, Brazil.

出版信息

Vet Parasitol. 2006 Nov 5;141(3-4):260-3. doi: 10.1016/j.vetpar.2006.05.017. Epub 2006 Jun 27.

DOI:10.1016/j.vetpar.2006.05.017

PMID:16806711

Abstract

The ELISA test was evaluated for the diagnosis of bovine cysticercosis using heterologous antigens from the larvae of T. solium and T. crassiceps, by using different types of positive and negative control sera, to allow a broader analysis of the results. The ELISA test showed low sensitivity under natural conditions of bovine cysticercosis manifestation, but high rates (up to 90%) under experimental conditions. The high specificity of the test (81-100%) made evident its capacity to differentiate cysticercosis from other bovine diseases. No difference in performance was found among the antigens studied. It was concluded that the ELISA test has deficiencies in detecting anti-cysticercosis antibodies of animals at slaughterhouse. However, it can be useful in detecting experimentally infected animals and differentiating cysticercosis from other bovine diseases.

摘要

使用来自猪带绦虫和肥胖带绦虫幼虫的异源抗原，通过使用不同类型的阳性和阴性对照血清，对酶联免疫吸附测定（ELISA）试验进行评估，以更全面地分析结果，用于牛囊尾蚴病的诊断。ELISA试验在牛囊尾蚴病自然发病条件下敏感性较低，但在实验条件下阳性率较高（高达90%）。该试验的高特异性（81%-100%）表明其能够将囊尾蚴病与其他牛病区分开来。在所研究的抗原之间未发现性能差异。得出的结论是，ELISA试验在检测屠宰场动物的抗囊尾蚴病抗体方面存在不足。然而，它可用于检测实验感染的动物，并将囊尾蚴病与其他牛病区分开来。