Sikasunge C S, Johansen M V, Willingham A L, Leifsson P S, Phiri I K
School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia.
Vet Parasitol. 2008 Nov 25;158(1-2):57-66. doi: 10.1016/j.vetpar.2008.08.014. Epub 2008 Aug 31.
The aim of this study was to assess the effect of treating Taenia solium infected pigs with oxfendazole (OFZ) on viability and clearance of cysticerci and the corresponding persistence of specific antibody isotypes (IgG(total), IgG1, IgG2 and IgA) and circulating cysticercal antigen (CCA). Antibody isotypes and CCA responses were measured by antibody-ELISA (Ab-ELISA) and antigen ELISA (Ag-ELISA), respectively. Correlations were made between antibodies, CCA and the total number of cysticerci enumerated at necropsy. Forty pigs with cysticercosis were randomly allocated into two groups: Treatment group (n=20) was treated with OFZ at 30 mg/kg orally while the treatment control group (n=20) was not treated. Five uninfected pigs served as negative controls. Pigs were killed at 1, 4, 8 and 26 weeks post-treatment (wkpt). Overall, the mean total cyst count in treated pigs was 2904+/-5397 (mean+/-S.D.) while in the controls it was 6235+/-6705. Mean cyst viability was 5+/-11% (mean+/-S.D.) and 97+/-4% in treated and control pigs, respectively. Results showed that OFZ killed muscular cysticerci over a period of 4 weeks but failed to kill cerebral cysticerci. Antibodies, CCA responses and clearance of dead cysts from the meat, depended on the cyst intensity of individual pigs at time of treatment since both antibody and CCA correlated with intensity of cysticerci at necropsy (r=0.441, P=0.005; r=0.654, P<0.001), respectively. IgG1 responses were the best indicator of treatment efficacy because they were predominant in both infected treated and control pigs and disappeared early after treatment. Both Ab/Ag-ELISA failed to detect cysts in the brain. Though dead cysticerci took some time (26 wkpt) to clear from the meat, treatment of porcine cysticercosis with OFZ should, in combination with other intervention measures be considered as an important, cost-effective measure in the control of taeniosis/cysticercosis.
本研究的目的是评估用奥芬达唑（OFZ）治疗感染猪带绦虫的猪对囊尾蚴的活力和清除率以及相应特异性抗体亚型（IgG（总）、IgG1、IgG2和IgA）和循环囊尾蚴抗原（CCA）持续存在情况的影响。抗体亚型和CCA反应分别通过抗体酶联免疫吸附测定（Ab-ELISA）和抗原酶联免疫吸附测定（Ag-ELISA）进行检测。对抗体、CCA与尸检时囊尾蚴总数之间进行了相关性分析。40头患有囊尾蚴病的猪被随机分为两组：治疗组（n = 20）口服30 mg/kg的OFZ进行治疗，而治疗对照组（n = 20）未接受治疗。5头未感染的猪作为阴性对照。在治疗后1、4、8和26周（wkpt）对猪实施安乐死。总体而言，治疗组猪的平均囊尾蚴总数为2904±5397（平均值±标准差），而对照组为6235±6705。治疗组和对照组猪的囊尾蚴平均活力分别为5±11%（平均值±标准差）和97±4%。结果表明，OFZ在4周内杀死了肌肉中的囊尾蚴，但未能杀死脑内的囊尾蚴。抗体、CCA反应以及肉中死囊尾蚴的清除情况，取决于治疗时个体猪的囊尾蚴感染强度，因为抗体和CCA与尸检时囊尾蚴的强度均具有相关性（r = 0.441，P = 0.）。
