Laboratório de Inspeção de Produtos de Origem Animal, Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Laboratório de Virologia Animal, Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
PLoS Negl Trop Dis. 2018 Apr 12;12(4):e0006371. doi: 10.1371/journal.pntd.0006371. eCollection 2018 Apr.
Bovine cysticercosis is a worldwide distributed zoonosis caused by the larval form of Taenia saginata present in bovine muscles. The diagnosis is based on the postmortem inspection at slaughterhouses and consists of the macroscopic visualization of lesions caused by cysticercosis in muscle sites. However, parasitized animals can pass unnoticed during sanitary inspection. Thus, the objective of this study was to characterize and evaluate the performance of different peptides from different regions of T. saginata for the cysticercosis diagnosis using enzyme-linked immunosorbent assay. We generated and evaluated a new recombinant protein chimera derived from the fusion of different peptides. We selected three distinct regions of T. saginata and predicted six peptides with antigenic potential (EP2-EP7). These peptides were analyzed individually and selected for generating a new chimeric recombinant protein. The new protein was termed rqTSA-25, and its performance rates were: 93.3% sensitivity (confidence interval (CI) = 76-98%), 95.3% specificity (CI = 82-99%), 93% positive predictive value (CI = 76-98%), 95% negative predictive value (CI = 82-99%), and 95% accuracy. In the immunoblot, this protein showed no false positive or false negative reaction. Thus, the use of rqTSA-25 is recommended for the diagnosis of bovine cysticercosis.
牛囊尾蚴病是一种全球性分布的人畜共患病，由牛肌肉中的带绦虫幼虫引起。诊断基于屠宰场的尸检，包括在肌肉部位观察由囊尾蚴病引起的病变的宏观可视化。然而，受感染的动物在卫生检查中可能会被忽视。因此，本研究的目的是使用酶联免疫吸附试验（ELISA）来鉴定和评估来自不同区域的不同绦虫（Taenia saginata）肽段在囊尾蚴病诊断中的性能。我们生成并评估了一种新的重组蛋白嵌合体，该嵌合体源自不同肽段的融合。我们选择了绦虫的三个不同区域，并预测了六个具有抗原潜力的肽段（EP2-EP7）。这些肽段分别进行了分析，并被选择用于生成新的嵌合重组蛋白。新蛋白被命名为 rqTSA-25，其性能率为：93.3%的敏感性（置信区间（CI）=76-98%）、95.3%的特异性（CI=82-99%）、93%的阳性预测值（CI=76-98%）、95%的阴性预测值（CI=82-99%）和 95%的准确度。在免疫印迹中，该蛋白未显示出假阳性或假阴性反应。因此，建议使用 rqTSA-25 来诊断牛囊尾蚴病。
