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免疫传感器的构建:硫醇附着层的组成和结构如何影响免疫传感器的效率?

Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?

作者信息

Briand Elisabeth, Salmain Michèle, Herry Jean-Marie, Perrot Hubert, Compère Chantal, Pradier Claire-Marie

机构信息

Laboratoire de Réactivité de Surface, UMR CNRS 7609, Université Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France.

出版信息

Biosens Bioelectron. 2006 Sep 15;22(3):440-8. doi: 10.1016/j.bios.2006.05.018. Epub 2006 Jun 27.

DOI:10.1016/j.bios.2006.05.018
PMID:16806887
Abstract

Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated.

摘要

基于将模型兔抗体固定在混合自组装单分子层上,并以蛋白A作为连接剂固定在金换能器上构建了免疫传感器,在每一步骤都通过调制偏振红外光谱(PM-IRRAS)进行表征,偶尔也通过原子力显微镜(AFM)和石英晶体微天平(QCM)进行表征。通过测试两种不同的包含11-巯基十一烷酸(MUA)以及癸硫醇(C9CH3)或巯基己醇(C6OH)的混合自组装单分子层,证明了抗体附着层的化学成分和结构对传感器性能的作用。

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