Department of Nanoengineering, University of California-San Diego, La Jolla, California 92093, USA.
Nano Lett. 2012 Jan 11;12(1):396-401. doi: 10.1021/nl203717q. Epub 2011 Dec 7.
New template-based self-propelled gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines, functionalized with the Concanavalin A (ConA) lectin bioreceptor, are shown to be extremely useful for the rapid, real-time isolation of Escherichia coli (E. coli) bacteria from fuel-enhanced environmental, food, and clinical samples. These multifunctional microtube engines combine the selective capture of E. coli with the uptake of polymeric drug-carrier particles to provide an attractive motion-based theranostics strategy. Triggered release of the captured bacteria is demonstrated by movement through a low-pH glycine-based dissociation solution. The smaller size of the new polymer-metal microengines offers convenient, direct, and label-free optical visualization of the captured bacteria and discrimination against nontarget cells.
新型基于模板的自推进金/镍/聚苯胺/铂（Au/Ni/PANI/Pt）微管状发动机，功能化的伴刀豆球蛋白 A（ConA）凝集素生物受体，被证明对于从燃料增强的环境、食品和临床样本中快速、实时分离大肠杆菌（E. coli）非常有用。这些多功能微管发动机将大肠杆菌的选择性捕获与聚合物药物载体颗粒的摄取结合在一起，提供了一种有吸引力的基于运动的治疗策略。通过穿过低 pH 甘氨酸基解离溶液来证明捕获细菌的触发释放。新型聚合物-金属微引擎的较小尺寸提供了方便、直接和无标记的光学可视化捕获的细菌和区分非靶细胞。
