Weng Xuexiang, Bi Hongyan, Liu Baohong, Kong Jilie
Department of Chemistry, Fudan University, Shanghai 200433, P. R. China.
Electrophoresis. 2006 Aug;27(15):3129-35. doi: 10.1002/elps.200500840.
A novel method of chiral separation based on protein-stationary phase immobilized in a poly(methyl methacrylate) microfluidic chip was developed. BSA conjugated with the shortened carboxylic single-walled carbon nanotubes (SWNTs) was employed as the chiral selector. Successful separation of tryptophan enantiomers was achieved in less than 70 s with a resolution factor of 1.35 utilizing a separation length of 32 mm. This is the first example of chiral separation based on SWNTs-BSA conjugates as stationary phase immobilized in microchip channel. The stability of the stationary phase in the channel was examined by microchip electrophoresis with laser-induced fluorescence detection. Factors that influenced the chiral separation resolution were examined. Under the optimized conditions, the proposed modified chip revealed adequate repeatability concerning run-to-run. These results show that the use of SWNTs-BSA conjugates within microfluidic channels hold great promise for a variety of analytical schemes.
开发了一种基于固定在聚甲基丙烯酸甲酯微流控芯片中的蛋白质固定相的新型手性分离方法。将与缩短的羧基单壁碳纳米管（SWNTs）共轭的牛血清白蛋白（BSA）用作手性选择剂。利用32mm的分离长度，在不到70秒的时间内成功分离了色氨酸对映体，分离因子为1.35。这是以固定在微芯片通道中的SWNTs-BSA共轭物为固定相进行手性分离的首个实例。通过激光诱导荧光检测的微芯片电泳检查了通道中固定相的稳定性。研究了影响手性分离分辨率的因素。在优化条件下，所提出的改良芯片显示出良好的批间重复性。这些结果表明，在微流控通道中使用SWNTs-BSA共轭物在各种分析方案中具有巨大潜力。
