Liu Xiaojun, Liu Xin, Liang Aiye, Shen Zheng, Zhang Yu, Dai Zhongpeng, Xiong Bohui, Lin Bingcheng
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, P. R. China.
Electrophoresis. 2006 Aug;27(15):3125-8. doi: 10.1002/elps.200500890.
We developed a microfluidic chip-affinity CE method based on indirect LIF detection to study protein-drug interactions. The interaction between heparin and BSA was quantitatively studied, as a model system. In our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor EOF. The electrophoretic mobility changes of BSA were measured, with various concentrations of heparin added to the running buffer. Each run was completed within 80 s. The binding constant was determined to be (1.24 +/- 0.05) x 10(3) M(-1), which was in good agreement with that reported in the literature.
我们开发了一种基于间接激光诱导荧光检测的微流控芯片亲和毛细管电泳方法来研究蛋白质-药物相互作用。作为一个模型系统，对肝素与牛血清白蛋白之间的相互作用进行了定量研究。在我们的方法中，选择荧光素钠作为背景，重蒸馏水作为标记物来监测电渗流。在运行缓冲液中加入不同浓度的肝素，测量牛血清白蛋白的电泳迁移率变化。每次运行在80秒内完成。测定的结合常数为(1.24±0.05)×10³ M⁻¹，与文献报道的结果吻合良好。
