Piehl Natalia, Ludwig Martin, Belder Detlev
Abteilung für Chromatographie, Max-Planck-Institut für Kohlenforschung, Mülheim an der Ruhr, Germany.
Electrophoresis. 2004 Nov;25(21-22):3848-52. doi: 10.1002/elps.200406028.
Fast chiral separation of DNS-amino acids could be realized using microchip electrophoresis with fluorescence detection. For this purpose, highly sulfated cyclodextrins (HS-gamma-CD) were used as chiral selectors enabling high selectivity. Even subsecond separation of DNS-tryptophan, DNS-norleucine, DNS-phenylalanine, DNS-methionine, and DNS-aspartic acid could be achieved. Baseline separation could be accomplished within 720 ms, which is the fastest separation of enantiomers reported to date. A more complex mixture consisting of three chiral DNS-amino acids could be separated within 3.3 s utilizing a separation length of only 7 mm and an electrical field strength of 2012 V/cm.
使用带有荧光检测的微芯片电泳可以实现DNS-氨基酸的快速手性分离。为此，高度硫酸化的环糊精（HS-γ-CD）被用作手性选择剂，从而实现了高选择性。甚至可以实现DNS-色氨酸、DNS-正亮氨酸、DNS-苯丙氨酸、DNS-甲硫氨酸和DNS-天冬氨酸的亚秒级分离。在720毫秒内可以实现基线分离，这是迄今为止报道的对映体最快分离。利用仅7毫米的分离长度和2012 V/cm的电场强度，由三种手性DNS-氨基酸组成的更复杂混合物可以在3.3秒内分离。
