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利用固定化pH梯度和质谱法对铁和硫氧化嗜酸氧化亚铁硫杆菌进行蛋白质组学和生物信息学分析。

Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry.

作者信息

Bouchal Pavel, Zdráhal Zbynek, Helánová Sárka, Janiczek Oldrich, Hallberg Kevin B, Mandl Martin

机构信息

Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.

出版信息

Proteomics. 2006 Aug;6(15):4278-85. doi: 10.1002/pmic.200500719.

Abstract

A comparative analysis of the protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. A newly developed protocol involving immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome sequence information was applied. This approach resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best A. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influenced by the growth type, and allowed for the detection of many physiologically important proteins. Among them were sulfate adenylyltransferase and sulfide dehydrogenase, which are involved in sulfate assimilation and sulfide metabolism, respectively. Many other proteins were related to important processes like cell attachment and electron transport. Co-migration of phosphate and sulfate transport proteins was also observed.

摘要

对在元素硫和亚铁上生长的氧化亚铁硫杆菌细胞的蛋白质组成进行了比较分析。应用了一种新开发的方案,该方案涉及固定化pH梯度、改进的蛋白质还原、质谱蛋白质鉴定和全基因组序列信息。这种方法在较宽的碱性pH范围内产生了1300多个蛋白点,这是迄今为止氧化亚铁硫杆菌蛋白质组的最佳分辨率。比较图像分析表明,蛋白质组受生长类型的显著影响,并能检测到许多生理上重要的蛋白质。其中包括分别参与硫酸盐同化和硫化物代谢的硫酸腺苷转移酶和硫化物脱氢酶。许多其他蛋白质与细胞附着和电子传递等重要过程有关。还观察到磷酸盐和硫酸盐转运蛋白的共迁移。

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