Novoselova E G, Cherenkov D A, Glushkova O V, Novoselova T V, Chudnovskiĭ V M, Iusupov V I, Fesenko E E
Biofizika. 2006 May-Jun;51(3):509-18.
The effect of in vitro exposure to low-power laser light with a power density of 0.2 mW/cm2 and a wavelength of 632.8 nm induced by helium-neon laser on the functional activity of macrophages and splenic lymphocytes was studied. If the exposure period did not exceed 60 sec, the stimulation in interleukin-2 (IL-2) and nitric oxide (NO) production, as well as an increase in the activity of natural killer cells were observed. The increase of irradiation dose by prolongation of the exposure duration up to 180 s induced a significant decrease in NO production and natural killer cell activity, but IL-2 production was not different from the control level. A remarkable decrease in interferon-gamma (IFN-gamma) production was observed following laser light exposure of cells for 60 or 180 s, whereas under lower doses (exposure for 5 or 30 sec) IFN-gamma production increased. Irradiation of isolated macrophages induced a significant stimulation of cellular tumor necrosis factor-alpha (TNF- alpha) production at all dboes used, and, what is more important, an enhancement in both TNF-a phaand interleukin-6 (IL-6) production was revealed as early as after a 5-s exposure. In this case, more prolonged exposure periods, 60 and 180 s, either did not induce changes in IL-6 production (in macrophages) or decreased IL-6 production (in lymphocytes). Thus, upon in vitro exposure of cells to extremely low-power laser light, a basic tendency was observed: short-term irradiation predominantly induced stimulation in secretory activity of cells, whereas prolongation of exposure mainly induced immunosuppression. The only exception to the rule was a change in interleukin-3 (IL-3) production, which decreased after short-time exposure, but, on the opposite, increased when the cells were exposed for 180 s. In addition, a high sensitivity to extremely low-power laser light was supported by expression of the inducible heat shock protein, Hsp70, the effect being observed at all doses used, including the exposure for 5 s. At the same time, expression of another heat shock protein, Hsp90, was somewhat reduced after irradiation of cells with laser light.
研究了体外暴露于氦氖激光诱导的功率密度为0.2 mW/cm²、波长为632.8 nm的低功率激光对巨噬细胞和脾淋巴细胞功能活性的影响。如果暴露时间不超过60秒，可观察到白细胞介素-2（IL-2）和一氧化氮（NO）产生的刺激以及自然杀伤细胞活性的增加。通过将暴露持续时间延长至180秒来增加照射剂量，会导致NO产生和自然杀伤细胞活性显著降低，但IL-2产生与对照水平无差异。细胞暴露于激光60秒或180秒后，干扰素-γ（IFN-γ）产生显著降低，而在较低剂量（暴露5秒或30秒）下IFN-γ产生增加。在所有使用的剂量下，对分离的巨噬细胞进行照射均能显著刺激细胞肿瘤坏死因子-α（TNF-α）的产生，更重要的是，早在5秒暴露后就发现TNF-α和白细胞介素-6（IL-6）的产生均增强。在这种情况下，更长时间的暴露，即60秒和180秒，要么不会诱导IL-6产生变化（在巨噬细胞中），要么会降低IL-6产生（在淋巴细胞中）。因此，细胞在体外暴露于极低功率激光时，观察到一个基本趋势：短期照射主要诱导细胞分泌活性的刺激，而延长暴露主要诱导免疫抑制。该规则的唯一例外是白细胞介素-3（IL-3）产生的变化，短时间暴露后其产生减少，但相反，细胞暴露180秒时其产生增加。此外，可诱导热休克蛋白Hsp70的表达支持了对极低功率激光的高敏感性，在所有使用的剂量下，包括暴露5秒时，均观察到这种效应。同时，细胞用激光照射后，另一种热休克蛋白Hsp90的表达有所降低。
