在毛细管区带电泳中用十二烷基三甲基溴化铵进行在线样品预富集。
On-line sample preconcentration by sweeping with dodecyltrimethylammonium bromide in capillary zone electrophoresis.
作者信息
Gong Maojun, Wehmeyer Kenneth R, Limbach Patrick A, Heineman William R
机构信息
Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA.
出版信息
J Chromatogr A. 2006 Sep 1;1125(2):263-9. doi: 10.1016/j.chroma.2006.05.058. Epub 2006 Jun 30.
On-line sample preconcentration of oligonucleotides with a new sweeping carrier was developed by using dodecyltrimethylammonium bromide (DTAB) below the critical micelle concentration (CMC). The sweeping results with DTAB below and above the CMC were compared. The use of DTAB below the CMC benefits the preconcentration of the oligonucleotides, while the use of DTAB above the CMC is good for hydrophobic small molecules. The factors affecting the sweeping results were optimized and this method was evaluated by constructing calibration curves for thrombin aptamers. The sweeping scheme produced a 112-fold sensitivity enhancement for the oligonucleotides relative to that run in a running buffer without DTAB. The sweeping method developed here can be a good reinforcement of the preconcentration scheme by sweeping when less-hydrophobic analytes or large negatively-charged molecules need to be preconcentrated.
通过使用低于临界胶束浓度（CMC）的十二烷基三甲基溴化铵（DTAB），开发了一种采用新型清扫载体对寡核苷酸进行在线样品预富集的方法。比较了低于和高于CMC的DTAB的清扫结果。在CMC以下使用DTAB有利于寡核苷酸的预富集，而在CMC以上使用DTAB则对疏水性小分子有利。优化了影响清扫结果的因素，并通过构建凝血酶适体的校准曲线对该方法进行了评估。与在没有DTAB的运行缓冲液中运行相比，该清扫方案使寡核苷酸的灵敏度提高了112倍。当需要预富集疏水性较小的分析物或带大量负电荷的分子时，此处开发的清扫方法可以很好地强化通过清扫进行的预富集方案。
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