Waltimo Tuomas, Zehnder Matthias, Söderling Eva
Institute of Oral Microbiology and Preventive Dentistry, University of Basel Center for Dental Medicine, Switzerland.
Acta Odontol Scand. 2006 Jun;64(3):183-6. doi: 10.1080/00016350500570700.
To assess whether bone powder in suspension enhances the antimicrobial efficacy of bioactive glass S53P4 against Gram-negative microbiota commonly associated with peri-implant disease.
Standardized suspensions of Porphyromonas gingivalis ATCC 33277 and YH 3, as well as Actinobacillus actinomycetemcomitans ATCC 29523 and KK 2 were added to 24-h suspensions of bioactive glass S53P4 with ground bovine bone powder, decalcified bone, or hydroxylapatite powder. Recovery of viable bacteria was assessed using anaerobic culture methods. As a reference, the antibacterial effect of an inert borosilicate powder with a particle size corresponding to that of the bioactive glass was tested. Counts of bacteria suspended in a pure unbuffered saline solution served as controls.
A significant drop in viable microorganisms was observed in suspensions of bioactive glass and bone powder compared to counterparts of pure bioactive glass. In contrast, neither the presence of hydroxylapatite powder nor the presence of decalcified bone in suspension caused any increase in bioactive glass killing efficacy on the microorganisms under investigation. Inert borosilicate glass showed no antibacterial effects per se or in combination with bone powder.
The antimicrobial effect of a combined bioactive glass-ground bone powder suspension was an in vitro observation which should be confirmed using adequate in vivo models.
评估悬浮液中的骨粉是否能增强生物活性玻璃S53P4对通常与种植体周围疾病相关的革兰氏阴性微生物群的抗菌效果。
将牙龈卟啉单胞菌ATCC 33277和YH 3以及伴放线放线杆菌ATCC 29523和KK 2的标准化悬浮液添加到含有磨碎的牛骨粉、脱钙骨或羟基磷灰石粉末的生物活性玻璃S53P4的24小时悬浮液中。使用厌氧培养方法评估活菌的回收率。作为对照，测试了一种粒径与生物活性玻璃相当的惰性硼硅酸盐粉末的抗菌效果。悬浮在纯无缓冲盐溶液中的细菌计数作为对照。
与纯生物活性玻璃悬浮液相比，生物活性玻璃和骨粉悬浮液中的活菌数量显著下降。相比之下，悬浮液中羟基磷灰石粉末的存在或脱钙骨的存在均未导致生物活性玻璃对所研究微生物的杀灭效果增加。惰性硼硅酸盐玻璃本身或与骨粉组合均未显示出抗菌效果。
生物活性玻璃-磨碎骨粉混合悬浮液的抗菌效果是一项体外观察结果，应使用适当的体内模型进行证实。
