Hoenen Thomas, Groseth Allison, Kolesnikova Larissa, Theriault Steven, Ebihara Hideki, Hartlieb Bettina, Bamberg Sandra, Feldmann Heinz, Ströher Ute, Becker Stephan
Institut für Virologie, Philipps Universität Marburg, Marburg, Germany.
J Virol. 2006 Jul;80(14):7260-4. doi: 10.1128/JVI.00051-06.
Infectious virus-like particle (iVLP) systems have recently been established for several negative-strand RNA viruses, including the highly pathogenic Zaire ebolavirus (ZEBOV), and allow study of the viral life cycle under biosafety level 2 conditions. However, current systems depend on the expression of viral helper nucleocapsid proteins in target cells, thus making it impossible to determine whether ribonucleoprotein complexes transferred by iVLPs are able to facilitate initial transcription, an indispensable step in natural infection. Here we describe a ZEBOV iVLP system which overcomes this limitation and show that VP24 is essential for the formation of a functional ribonucleoprotein complex.
最近，针对包括高致病性扎伊尔埃博拉病毒（ZEBOV）在内的几种负链RNA病毒建立了感染性病毒样颗粒（iVLP）系统，该系统可在生物安全2级条件下研究病毒生命周期。然而，目前的系统依赖于在靶细胞中表达病毒辅助核衣壳蛋白，因此无法确定iVLPs转移的核糖核蛋白复合物是否能够促进初始转录，而初始转录是自然感染中不可或缺的一步。在此，我们描述了一种克服这一局限性的ZEBOV iVLP系统，并表明VP24对于功能性核糖核蛋白复合物的形成至关重要。
