Filovirus Laboratory, INSERM U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France.
J Infect Dis. 2011 Nov;204 Suppl 3:S892-6. doi: 10.1093/infdis/jir311.
The structural protein VP24 of Ebola virus (EBOV) is a determinant of virulence in rodent models and possesses an interferon antagonist function. In this study, we investigate the role of VP24 in EBOV replication using RNA interference by small interfering RNA to knock down the expression of this protein in virus-infected cells. We reveal that VP24 is required for assembly of viral nucleocapsids and that silencing of VP24 expression prevents the release of EBOV.
埃博拉病毒（EBOV）的结构蛋白 VP24 是啮齿动物模型中病毒毒力的决定因素，具有干扰素拮抗剂功能。在这项研究中，我们通过 RNA 干扰（小干扰 RNA）来研究 VP24 在 EBOV 复制中的作用，从而敲低感染细胞中该蛋白的表达。我们揭示了 VP24 是病毒核衣壳组装所必需的，并且沉默 VP24 的表达可阻止 EBOV 的释放。
