Zielske Steven P, Stevenson Mario
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA.
J Virol. 2006 Jul;80(14):7275-80. doi: 10.1128/JVI.02470-05.
LEDGFp75 is a cellular protein which binds human immunodeficiency virus type 1 (HIV-1) integrase with high specificity and affinity but whose function in infection has not been defined. We infected LEDGFp75-deficient primary macrophages with wild-type HIV in order to assess potential infection phenotypes which would provide clues to LEDGFp75 function. Silencing of LEDGFp75 by 70 to 80% resulted in an average of 53% reduced infection of macrophages by HIV. Analysis of infection intermediates showed that integration, but not two-long-terminal-repeat (2LTR) circles or late cDNAs, was reduced up to 74% in LEDGFp75-deficient macrophages. Therefore, LEDGFp75 has a modest involvement in HIV-1 integration in macrophages.
LEDGFp75是一种细胞蛋白，它能以高特异性和亲和力与人免疫缺陷病毒1型（HIV-1）整合酶结合，但其在感染过程中的功能尚未明确。我们用野生型HIV感染LEDGFp75缺陷的原代巨噬细胞，以评估可能的感染表型，这些表型可为LEDGFp75的功能提供线索。LEDGFp75沉默70%至80%导致HIV对巨噬细胞的感染平均减少53%。对感染中间体的分析表明，在LEDGFp75缺陷的巨噬细胞中，整合过程（而非双长末端重复序列（2LTR）环或晚期cDNA）减少了74%。因此，LEDGFp75在巨噬细胞的HIV-1整合过程中起适度作用。
