Palma Beatriz Duarte, Gabriel Alexandre, Colugnati Fernando A B, Tufik Sergio
Departamento de Psicobiologia, Universidade Federal de São Paulo, Rua Botucatu, 862-1 andar, 04023-062 Vila Clementino, São Paulo, Brazil.
Am J Physiol Regul Integr Comp Physiol. 2006 Nov;291(5):R1527-32. doi: 10.1152/ajpregu.00186.2006. Epub 2006 Jun 29.
Sleep is hypothesized to play a restorative role on immune system. In addition, disturbed sleep is thought to impair host defense mechanisms. Chronic sleep deprivation is a common occurrence in modern society and has been observed in a number of chronic inflammatory conditions, such as systemic lupus erythematosus (SLE). New Zealand Black/New Zealand White (NZB/NZW) F1 mice develop an autoimmune disease that strongly resembles SLE in humans, exhibiting high titers of antinuclear antibodies associated with the development of rapidly progressive and lethal glomerulonephritis. On the basis of this evidence, the present study examined the onset and progress of lupus in as-yet healthy female mice submitted to sleep deprivation. Sleep deprivation was accomplished by two 96-h periods in the multiple-platform method when mice were 10 wk old, and they were observed until 28 wk of age. Blood samples were collected from the orbital plexus fortnightly to evaluate serum antinuclear antibodies and anti-double-stranded DNA. Proteinuria and longevity as well as body weight were also assessed. The results indicated that mice submitted to sleep deprivation exhibited an earlier onset of the disease, as reflected by the increased number of antinuclear antibodies. However, no statistical difference was found in the other parameters analyzed. According to these results, sleep deprivation could be considered as a risk factor for the onset but not for the evolution of the disease.
据推测，睡眠对免疫系统具有恢复作用。此外，睡眠障碍被认为会损害宿主防御机制。慢性睡眠剥夺在现代社会很常见，并且在一些慢性炎症性疾病中也有观察到，如系统性红斑狼疮（SLE）。新西兰黑/新西兰白（NZB/NZW）F1小鼠会患上一种与人类SLE极为相似的自身免疫性疾病，表现出高滴度的抗核抗体，这些抗体与快速进展性和致死性肾小球肾炎的发展有关。基于这一证据，本研究检查了尚未患病的雌性小鼠在遭受睡眠剥夺后狼疮的发病和进展情况。当小鼠10周龄时，采用多平台方法通过两个96小时的时间段来实现睡眠剥夺，并对它们进行观察直至28周龄。每两周从眶静脉丛采集血样以评估血清抗核抗体和抗双链DNA。还评估了蛋白尿、寿命以及体重。结果表明，遭受睡眠剥夺的小鼠疾病发病更早，这可通过抗核抗体数量的增加反映出来。然而，在分析的其他参数中未发现统计学差异。根据这些结果，睡眠剥夺可被视为疾病发病的一个风险因素，但不是疾病进展的风险因素。
