Blanco Flavio Antonio, Zanetti María Eugenia, Casalongué Claudia Anahí, Daleo Gustavo Raúl
Instituto de Investigaciones Biológicas-Departamento de Biología, Universidad Nacional de Mar del Plata, Funes 3250, CC 1245, 7600 Mar del Plata, Argentina.
Plant Physiol Biochem. 2006 May-Jun;44(5-6):315-22. doi: 10.1016/j.plaphy.2006.05.005. Epub 2006 Jun 16.
The MAPK cascade is an evolutionary conserved signaling pathway that links external stimuli with cellular responses. Using polymerase chain reaction (PCR), a DNA fragment corresponding to a Solanum tuberosum MAPK, StMPK1, was isolated. StMPK1 amino acid sequence displayed over 90% identity with tomato MPK1 (LeMPK1) and tobacco SIPK. Southern blot analysis indicated that the gene encoding StMPK1 is present in a single copy in the potato genome. StMPK1 mRNA levels differentially accumulated in potato tuber in response to wounding and to wounding plus Fusarium solani f. sp. eumartii. Transcript accumulation after infection was transient and started earlier than what was observed in wounded tubers. StMPK1 mRNA levels also increased in potato tuber after 24 h of treatment with jasmonic acid (JA) and abscicic acid (ABA), but not in response to ethylene or salicylic acid. In addition, StMPK1 transcript levels increased after a heat-shock treatment at 42 degrees C. The results suggest that StMPK1 may participate in the cellular responses against multiple environmental stimuli in potato tubers.
丝裂原活化蛋白激酶（MAPK）级联反应是一条进化保守的信号通路，它将外部刺激与细胞反应联系起来。利用聚合酶链反应（PCR），分离出了一个与马铃薯MAPK（StMPK1）相对应的DNA片段。StMPK1氨基酸序列与番茄MPK1（LeMPK1）和烟草SIPK的同源性超过90%。Southern杂交分析表明，编码StMPK1的基因在马铃薯基因组中以单拷贝形式存在。StMPK1 mRNA水平在马铃薯块茎中因受伤以及受伤加茄腐镰孢菌而差异积累。感染后的转录积累是短暂的，且比在受伤块茎中观察到的开始得更早。用茉莉酸（JA）和脱落酸（ABA）处理24小时后，马铃薯块茎中的StMPK1 mRNA水平也有所增加，但对乙烯或水杨酸无反应。此外，在42℃热激处理后，StMPK1转录水平增加。结果表明，StMPK1可能参与马铃薯块茎对多种环境刺激的细胞反应。
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