在希腊塞尔迈湾发生腹泻性贝类中毒（DSP）事件期间，采用生物学、化学和免疫学方法检测贻贝中的海洋毒素冈田酸。
Detection of the marine toxin okadaic acid in mussels during a diarrhetic shellfish poisoning (DSP) episode in Thermaikos Gulf, Greece, using biological, chemical and immunological methods.
作者信息
Mouratidou Theoni, Kaniou-Grigoriadou Ignatia, Samara Constantini, Kouimtzis Themistokles
机构信息
National Reference Laboratory on Marine Biotoxins, Center of Veterinary Institutions of Thessaloniki, Ministry of Agriculture, Limnou 3A, 546 27 Thessaloniki, Greece.
出版信息
Sci Total Environ. 2006 Aug 1;366(2-3):894-904. doi: 10.1016/j.scitotenv.2005.03.002. Epub 2006 Jul 3.
An approach involving chemical and biological techniques was taken for the detection and quantification of the marine toxin okadaic acid (OA) in mussels from Thermaikos Gulf, Greece, during a 4-month DSP episode that occurred in 2002. Samples were analyzed using the mouse bioassay, high performance liquid chromatography (HPLC) with fluorimetric detection and an enzyme-linked immunosorbent assay (ELISA). Okadaic acid was quantifiable at three different sites of Thermaikos Gulf reaching a maximum concentration of 36 microg/g hepatopancreas. High correlation was revealed between results derived from HPLC and ELISA (R2=0.998), while 91% consistency between HPLC and the mouse bioassay results was observed.
在2002年发生的一次为期4个月的腹泻性贝类中毒事件期间，采用化学和生物技术相结合的方法对希腊塞尔迈湾贻贝中的海洋毒素冈田酸（OA）进行检测和定量。使用小鼠生物测定法、荧光检测高效液相色谱法（HPLC）和酶联免疫吸附测定法（ELISA）对样品进行分析。在塞尔迈湾的三个不同地点均可检测到冈田酸，肝胰腺中最高浓度达到36微克/克。HPLC和ELISA的结果显示出高度相关性（R2 = 0.998），同时观察到HPLC与小鼠生物测定结果之间的一致性为91%。
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