Prassopoulou Eleanna, Katikou Panagiota, Georgantelis Dimitrios, Kyritsakis Apostolos
Department of Food Technology, Technological Educational Institute of Thessaloniki, 54110 Sindos, Greece.
Toxicon. 2009 Feb;53(2):214-27. doi: 10.1016/j.toxicon.2008.11.003. Epub 2008 Nov 18.
An approach involving chemical, functional and biological techniques was taken for the detection and quantification of the marine toxin okadaic acid (OA) in mussels from Thermaikos and Saronikos Gulfs, Greece, during DSP episodes that occurred in 2006-2007. Samples were analyzed using the mouse bioassay, high performance liquid chromatography with fluorimetric detection (HPLC-FLD), using l-bromoacetylpyrene (BAP), as a precolumn derivatisation reagent, and the protein phosphatase 2A inhibition assay (PP2AIA) using a commercially available kit. Okadaic acid (OA) and its polar and non-polar esters were detected and quantified by HPLC-FLD, after hydrolysis of the samples during preparation. The detection limit of the HPLC method for OA was 5.86 microg OA/kg, which permits this method to be used for the regulatory control of these toxins in shellfish. Comparison of the results by all three methods revealed excellent consistency.
在2006 - 2007年发生腹泻性贝类中毒（DSP）事件期间，采用化学、功能和生物技术相结合的方法，对希腊塞尔迈湾和萨罗尼科斯湾贻贝中的海洋毒素冈田酸（OA）进行检测和定量分析。样品分别采用小鼠生物测定法、使用1 - 溴乙酰芘（BAP）作为柱前衍生试剂的荧光检测高效液相色谱法（HPLC - FLD）以及使用市售试剂盒的蛋白磷酸酶2A抑制测定法（PP2AIA）进行分析。在样品制备过程中进行水解后，通过HPLC - FLD对冈田酸（OA）及其极性和非极性酯进行检测和定量。OA的HPLC方法检测限为5.86微克OA/千克，这使得该方法可用于贝类中这些毒素的监管控制。三种方法的结果比较显示出极好的一致性。
