一种新型含KRAB结构域的锌指基因（ZNF284L）的克隆与鉴定
Cloning and characterization of a novel KRAB-domain-containing zinc finger gene (ZNF284L).
作者信息
Yin Gang, Ji Chaoneng, Zeng Li, Wang Zebin, Wang Jiayan, Shen Zhouliang, Wu Tong, Gu Shaohua, Xie Yi, Mao Yumin
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China.
出版信息
Mol Biol Rep. 2006 Jun;33(2):137-44. doi: 10.1007/s11033-005-6405-3.
The zinc finger gene (ZNF) family plays an important role in the regulation of transcription. This study reports the cloning and characterization of a novel human zinc finger protein cDNA (ZNF284L) from fetal brain cDNA library. The ZNF284L cDNA is 2223 bp in length encoding a 593-aa polypeptide. The protein contains a KRAB A+b box and eleven C2H2 type zinc finger motifs. ZNF284L gene is mapped to 19q13.2-19q13.3 with 5 exons, and the expression pattern of ZNF284L gene was also examined by reverse transcription polymerase chain reaction (RT-PCR). The transcripts were detected in the human lung, liver, pancreas, thymus, heart, placenta, spleen, prostate, ovary, small intestine and colon, but in human brain, skeletal muscle, kidney, testis and peripheral blood leukocyte, no expression was detected.
锌指基因（ZNF）家族在转录调控中发挥着重要作用。本研究报告了从胎儿脑cDNA文库中克隆并鉴定出一种新型人类锌指蛋白cDNA（ZNF284L）。ZNF284L cDNA长度为2223 bp，编码一个593个氨基酸的多肽。该蛋白包含一个KRAB A + b盒和11个C2H2型锌指基序。ZNF284L基因定位于19q13.2 - 19q13.3，有5个外显子，并且还通过逆转录聚合酶链反应（RT-PCR）检测了ZNF284L基因的表达模式。在人类肺、肝、胰腺、胸腺、心脏、胎盘、脾脏、前列腺、卵巢、小肠和结肠中检测到了转录本，但在人类脑、骨骼肌、肾脏、睾丸和外周血白细胞中未检测到表达。
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