Wu H, Zhang S, Qiu W, Zhang G, Xia Q, Xiao C, Huang X, Huang M, Agen P, Fan T, Yang J, Milunsky A
Department of Medical Genetics, First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan 610041, PR China.
Biochim Biophys Acta. 2001 Mar 19;1518(1-2):190-3. doi: 10.1016/s0167-4781(01)00172-5.
A novel human KRAB (Krüppel associated box) type zinc finger protein encoding gene, ZNF463, was obtained by mRNA differential display and RACE. It consists of 1904 nucleotides and encodes a protein of 463 amino acids with an amino-terminal KRAB domain and 12 carboxy-terminal C2H2 zinc finger units. The gene is mapped to chromosome 19q13.3 approximately 4 by FISH. As from Northern blot analysis ZNF463 is only expressed in testis, RT-PCR indicates that ZNF463 is expressed more highly in normal fertile adults than in fetus and azoospermic patients suggesting that it may play a role in human spermatogenesis.
通过mRNA差异显示和RACE技术获得了一个新的人类KRAB（Krüppel相关盒）型锌指蛋白编码基因ZNF463。它由1904个核苷酸组成，编码一个含有氨基末端KRAB结构域和12个羧基末端C2H2锌指单元的463个氨基酸的蛋白质。通过荧光原位杂交（FISH）将该基因定位到19号染色体q13.3区域约4处。Northern印迹分析表明ZNF463仅在睾丸中表达，而逆转录-聚合酶链反应（RT-PCR）显示，ZNF463在正常生育的成年人中的表达高于胎儿和无精子症患者，这表明它可能在人类精子发生过程中发挥作用。
