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本文引用的文献

1
Comparison of four methods to enumerate probiotic bifidobacteria in a fermented food product.四种方法用于对发酵食品中益生菌双歧杆菌进行计数的比较。
Food Microbiol. 2006 Sep;23(6):571-7. doi: 10.1016/j.fm.2005.09.001. Epub 2005 Oct 26.
2
A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester.一种用内部缀合的荧光探针 5（和 6-）-羧基荧光素琥珀酰亚胺酯连续测定细菌细胞内 pH 的新方法。
Appl Environ Microbiol. 1996 Jan;62(1):178-83. doi: 10.1128/aem.62.1.178-183.1996.
3
Probiotic bacteria may become dormant during storage.益生菌在储存期间可能会进入休眠状态。
Appl Environ Microbiol. 2005 Mar;71(3):1662-3. doi: 10.1128/AEM.71.3.1662-1663.2005.
4
The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state.多参数流式细胞术在监测单个微生物细胞生理状态中的应用。
Adv Biochem Eng Biotechnol. 2004;89:197-223. doi: 10.1007/b93997.
5
Survival of Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum BB-02 in white-brined cheese.嗜酸乳杆菌LA-5和两歧双歧杆菌BB-02在白盐渍奶酪中的存活情况。
Int J Food Sci Nutr. 2004 Feb;55(1):53-60. doi: 10.1080/09637480310001642484.
6
Effect of milk base and starter culture on acidification, texture, and probiotic cell counts in fermented milk processing.乳基和发酵剂对发酵乳加工过程中酸化、质地及益生菌细胞数量的影响。
J Dairy Sci. 2002 Oct;85(10):2479-88. doi: 10.3168/jds.S0022-0302(02)74330-0.
7
Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress.多参数流式细胞术和细胞分选用于评估胆汁盐胁迫期间双歧杆菌活细胞、受损细胞和死细胞
Appl Environ Microbiol. 2002 Nov;68(11):5209-16. doi: 10.1128/AEM.68.11.5209-5216.2002.
8
Development of a flow cytometric method to analyze subpopulations of bacteria in probiotic products and dairy starters.一种用于分析益生菌产品和乳制品发酵剂中细菌亚群的流式细胞术方法的开发。
Appl Environ Microbiol. 2002 Jun;68(6):2934-42. doi: 10.1128/AEM.68.6.2934-2942.2002.
9
Rapid fluorescence assessment of intracellular pH as a viability indicator of Clavibacter michiganensis subsp. michiganensis.作为密执安棒杆菌密执安亚种活力指标的细胞内pH值的快速荧光评估
J Appl Microbiol. 2000 May;88(5):809-16. doi: 10.1046/j.1365-2672.2000.01014.x.
10
Fluorescence assessment of Lactococcus lactis viability.乳酸乳球菌活力的荧光评估。
Int J Food Microbiol. 2000 Apr 10;55(1-3):291-4. doi: 10.1016/s0168-1605(00)00170-7.

通过流式细胞术活力测定法确定的所谓休眠益生菌的内在特性。

Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays.

作者信息

Lahtinen Sampo J, Ouwehand Arthur C, Reinikainen Johanna P, Korpela Jaakko M, Sandholm Jouko, Salminen Seppo J

机构信息

Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland.

出版信息

Appl Environ Microbiol. 2006 Jul;72(7):5132-4. doi: 10.1128/AEM.02897-05.

DOI:10.1128/AEM.02897-05
PMID:16820522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1489320/
Abstract

Plate counting and four culture-independent flow cytometric assays were used to determine the viability and intrinsic properties of three probiotic strains during storage. The strains showed reduction in plate counts but were able to maintain esterase activity, intact cytoplasmic membrane, and pH gradient. The apparently uncultivable probiotic cells were active and stress resistant.

摘要

采用平板计数法和四种非培养性流式细胞术检测方法，以确定三种益生菌菌株在储存期间的活力和内在特性。这些菌株的平板计数有所减少，但能够维持酯酶活性、完整的细胞质膜和pH梯度。明显不可培养的益生菌细胞具有活性且抗应激。