Koyama Tomohiro, Hirai Takuya, Nagai Shinya
Nippon Institute for Biological Science, Tokyo, Japan.
J Vet Med Sci. 2006 Jun;68(6):609-13. doi: 10.1292/jvms.68.609.
Lawsonia intracellularis isolated from a Japanese field case of porcine proliferative enteropathy (PPE) was cultivated and partially characterized. The bacterial cells isolated from the intestinal mucosa of a pig suffering from the acute form of PPE were used to inoculate rat small intestine cells (IEC-18) and human epithelial cells (HEp-2). Infected foci, which were stained with L. intracellularis-specific rabbit antiserum, were observed in the cell culture at 5 days post inoculation. The DNA sequence of several genes in the Japanese isolate had high similarity with those of the L. intracellularis type strain, suggesting the genetically close relationship of the two strains. This is the first report describing the cultivation and partial characterization of L. intracellularis originated in Japan.
从日本一例猪增生性肠炎（PPE）田间病例中分离出胞内劳森菌，并对其进行培养和部分特性鉴定。从患有急性PPE的猪的肠黏膜中分离出的细菌细胞被用于接种大鼠小肠细胞（IEC-18）和人上皮细胞（HEp-2）。接种后5天，在细胞培养物中观察到用胞内劳森菌特异性兔抗血清染色的感染灶。日本分离株中几个基因的DNA序列与胞内劳森菌模式菌株的序列高度相似，表明这两个菌株在遗传上关系密切。这是首次报道源自日本的胞内劳森菌的培养和部分特性鉴定。
