Watarai Masahisa, Yoshiya Masahiro, Sato Akane, Furuoka Hidefumi
Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan.
J Vet Med Sci. 2008 Jul;70(7):731-3. doi: 10.1292/jvms.70.731.
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. In this study, we improved the in vitro cultivation method of L. intracellularis to increase the passage efficiency and showed that L. intracellularis isolated from a rabbit and a pig have different antigenic properties. Bacteria should be recovered from infected cells before cell death due to infection to obtain higher bacterial passage efficiency, and measurement of LDH activity in the cell culture medium was useful for determining the timing of bacterial passage. L. intracellularis isolated from the rabbit and pig showed different band patterns in immunoblotting. Our results should be helpful in the development of serological diagnosis and epidemiological investigation methods.
胞内劳森菌是一种专性胞内致病细菌，可引起家畜和实验动物的增生性肠炎。在本研究中，我们改进了胞内劳森菌的体外培养方法以提高传代效率，并表明从兔和猪分离出的胞内劳森菌具有不同的抗原特性。为了获得更高的细菌传代效率，应在感染导致细胞死亡之前从感染细胞中回收细菌，并且测量细胞培养基中的乳酸脱氢酶活性有助于确定细菌传代的时机。从兔和猪分离出的胞内劳森菌在免疫印迹中显示出不同的条带模式。我们的结果应有助于血清学诊断和流行病学调查方法的发展。
