Beller Mathias, Oliver Brian
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Chromosome Res. 2006;14(4):349-62. doi: 10.1007/s10577-006-1065-2.
From the beginning, Drosophila was a high-throughput model organism. Unbiased and genome-wide efforts ranging from Morgan's search for spontaneous mutations and subsequent saturating loss-of-function and gain-of-function screens up to more recent techniques such as microarrays, proteomics and cellular assays have been and will continue to be the backbone of Drosophila research. Integrating these large datasets is one of the next challenges. However, once achieved, a plethora of information far exceeding the information content of the singular experiments will be revealed. Several high-throughput techniques and experimental strategies highlighting the unbiased and integrative nature of Drosophila research during the last century will be discussed.
从一开始，果蝇就是一种高通量模式生物。从摩根对自发突变的搜寻以及随后的功能丧失和功能获得的饱和筛选，到诸如微阵列、蛋白质组学和细胞分析等最新技术，无偏见的全基因组研究一直是且将继续是果蝇研究的支柱。整合这些大型数据集是接下来的挑战之一。然而，一旦成功，将会揭示出大量远超单个实验信息内容的信息。将讨论几种在上个世纪突出果蝇研究无偏见和综合性本质的高通量技术及实验策略。
