Milewicz Tomasz, Krzysiek Józef, Sztefko Krystyna, Radowicki Stanisław, Krzyczkowska-Sendrakowska Magdalena
Department of Gynecological Endocrinology Jagiellonian University, and Department of Clinical Biochemistry, Universtiy Children Hospital, Cracow, Poland.
Endokrynol Pol. 2005 Nov-Dec;56(6):876-82.
Ovarian hormonal function may be as important contributing factor to hGH-IGF-I-IGFBP-3 axis as age.
To examine plasma hGH, IGF-1 and IGFBP-3 levels in women with premature ovarian failure compared to healthy normal controls and postmenopausal ones.
Group A-15 women with premature ovarian failure (POF) (mean: age 38.9+/-5.2 years, FSH 101.4+/-29.0 IU/l; 17beta-estradiol 22.5+/-14.6 ng/l). Group B consisted of 15 menopausal women (mean: age 54.7+/-2.7 years; FSH 81.9+/-32.1 IU/l; 17beta-estradiol 17.1+/- 8.0 ng/l). Group C - controls - 15 normally menstruating women (mean: age 37.1+/-9.0 years; FSH 6.2+/-1.0 IU/l; 17beta-estradiol 144.8+/-117.1 ng/l).
Body mass and BMI were measured. Basic fasting plasma hGH, IGF-I, IGFBP-3, insulin, testosterone and LH as well as prolactin (PRL), FSH and estradiol were assessed by RIA kits. Statistical analysis. Shapiro-Wilk test, Mann-Whitney u-test, Spearman rang correlation coefficient, stepwise multiple regression.
Mean serum IGF-I level was the lowest (p<0.005) in group B (172.0+/-54.6 microg/l) and the highest in group C (273.6+/-109.0 microg/l). The mean plasma IGF-I level in group A was similar (NS) (208.3+/-66.5 microg/l) to that found in group B and lower (p<0.02) compared with that in group C. The lowest (p<0.005) serum IGFBP-3 level was found in group B (3.1+/-0.7 microg/l) compared to group C (4.4+/-0.3 microg/l). The mean plasma IGFBP-3 level (3.1+/-1.0 microg/l) in group A was lower than in group C (p<0.005) but identical as in group B. No statistically significant differences between groups were observed in mean hGH levels. Women in group A and C were younger (p<0.001) than those in group B. The lowest mean estradiol level was found in groups A and B. The highest was in group C (p<0.001). Mean plasma LH and FSH levels were higher (p<0.001) in groups A and B vs group C. In group C there were links between IGF-I and age (r=-0.60; p=0.014) The IGF-I/age relation disappeared in the groups A and B (rA=-0.26; rB=0.10; NS). The same regards IGFBP-3/ age link (rA=-0.44, NS; rB=0,31;NS). Estradiol level was related to hGH levels in group C (r=-0.54; p<0.05). In none of groups hGH/IGF-1 as well as IGFBP-3/hGH relations were found. Prolactin accounted for 69% of the variance in IGF-I level in the group B (p=0.003) and for 24% in group A (NS). Testosterone accounted for 88% (p=0.004) of the variance in IGF-I level in group B and IGFBP-3 was responsible for 86% (p=0.038) of the variance in IGF-I level in group C. Again IGFBP-3 was responsible for 47% (p=0.023) in group A and for 49% (p=0.04) in group B of the hGH variance.
17b-estradiol may be as important contributor to insulin-like growth factor-I (IGF-I) plasma level as age in hypoestrogenic, hypogonadotropic women.
卵巢激素功能可能与年龄一样，是生长激素-胰岛素样生长因子-I-胰岛素样生长因子结合蛋白-3轴的重要影响因素。
比较卵巢早衰女性与健康正常对照者及绝经后女性的血浆生长激素、胰岛素样生长因子-1（IGF-1）和胰岛素样生长因子结合蛋白-3（IGFBP-3）水平。
A组-15例卵巢早衰（POF）女性（平均年龄：38.9±5.2岁，促卵泡生成素（FSH）101.4±29.0IU/L；17β-雌二醇22.5±14.6ng/L）。B组由15例绝经后女性组成（平均年龄：54.7±2.7岁；FSH81.9±32.1IU/L；17β-雌二醇17.1±8.0ng/L）。C组-对照组-15例月经正常女性（平均年龄：37.1±9.0岁；FSH6.2±1.0IU/L；17β-雌二醇144.8±117.1ng/L）。
测量体重和体重指数（BMI）。采用放射免疫分析试剂盒评估基础空腹血浆生长激素、IGF-1、IGFBP-3、胰岛素、睾酮、促黄体生成素（LH）以及催乳素（PRL）、FSH和雌二醇。统计分析。采用夏皮罗-威尔克检验、曼-惠特尼U检验、斯皮尔曼等级相关系数、逐步多元回归分析。
B组平均血清IGF-1水平最低（p<0.005）（172.0±54.6μg/L），C组最高（273.6±109.0μg/L）。A组平均血浆IGF-1水平（208.3±66.5μg/L）与B组相似（无统计学差异），与C组相比更低（p<0.02）。与C组（4.4±0.3μg/L）相比，B组血清IGFBP-3水平最低（p<0.005）。A组平均血浆IGFBP-3水平（3.1±1.0μg/L）低于C组（p<0.005），但与B组相同。各组间平均生长激素水平未观察到统计学显著差异。A组和C组女性比B组年轻（p<0.001）。A组和B组雌二醇平均水平最低，C组最高（p<0.001）。A组和B组平均血浆LH和FSH水平高于C组（p<0.001）。在C组中，IGF-1与年龄相关（r=-0.60；p=0.014）。在A组和B组中，IGF-1/年龄关系消失（rA=-0.26；rB=0.10；无统计学差异）。IGFBP-3/年龄关系同样如此（rA=-0.44，无统计学差异；rB=0.31；无统计学差异）。在C组中，雌二醇水平与生长激素水平相关（r=-0.54；p<0.05）。在任何一组中均未发现生长激素/IGF-1以及IGFBP-3/生长激素之间的关系。在B组中，催乳素占IGF-1水平变异的69%（p=0.003），在A组中占24%（无统计学差异）。在B组中，睾酮占IGF-1水平变异的88%（p=0.004），在C组中IGFBP-3占IGF-1水平变异的86%（p=0.038）。同样，在A组中IGFBP-3占生长激素变异的47%（p=0.023），在B组中占49%（p=0.04）。
在雌激素缺乏、性腺功能减退的女性中，17β-雌二醇可能与年龄一样，是胰岛素样生长因子-I（IGF-I）血浆水平的重要影响因素。
