Milewicz Tomasz, Krzysiek Józef, Rogatko Iwona, Sztefko Krystyna, Stochmal Ewa, Jach Robert, Galicka-Latała Danuta, Radowicki Stanislaw, Huras Hubert, Radoń-Pokracka Małgorzata
Department of Gynecological Endocrinology, Collegium Medicum, Jagiellonian University, Cracow, Poland.
Neuro Endocrinol Lett. 2011;32(6):857-64.
To compare the influence of low and normal endogenous estradiol concentration on circulating hGH, IGF-I and IGFBP-3 levels as well as on mutual correlations of these parameters.
45 women (age 30.7 ± 9.0 years, BMI 25.7 ± 8.0) divided into group A - 15 hypoestrogenic women and group B - 30 normoestrogenic controls. Neither of the women was menopausal nor hyperprolactinemic.
Blood sample was taken at the standard conditions prior to the initiation of hormonal supplementation therapy in group A and at the day 3-5 of menstrual cycle in group B. Serum hGH, IGF-I, IGFBP-3, insulin, testosterone, sex hormone binding globulin (SHBG) dihydroepiandrosterone sulphate (DHEAS) and LH as well as prolactin (PRL), FSH and estradiol levels were measured by standard RIA kits.
Mean IGF-1, LH, FSH, testosterone and estradiol and PRL plasma levels were lower in group A compared to group B. There were no significant differences in mean SHBG, insulin and DHEAS levels. There were also no differences in mean: age, body mass, BMI as well as percentage of each BMI range between groups. Regardless the estradiol level the IGF-I/age link was found in both groups. A IGF-I/IGFBP-3 relation was found in both groups. IGF-I/estradiol link was seen only in group A. In group B hGH/SHBG link was found, in group A this relation was indirect. A link between hGH and testosterone levels was found only in group B. SHBG was related in group B to IGFBP-3, testosterone and to DHEAS. Insulin/IGFBP-3 link was seen in group B. The stepwise multiple regression revealed DHEAS and LH as predictors of IGF-I level in group A, while in group B none of the parameters predicted IGF-I level. The results of the same analysis in case of hGH are as follows: in group A hGH level was predicted by estradiol and SHBG. In group B none of factors predicted hGH levels.
Estradiol plasma level is correlated to circulating IGF-I, albeit the relation seems to be biphasic.
比较内源性雌二醇低浓度和正常浓度对循环中生长激素（hGH）、胰岛素样生长因子-I（IGF-I）和胰岛素样生长因子结合蛋白-3（IGFBP-3）水平的影响，以及对这些参数相互关系的影响。
45名女性（年龄30.7±9.0岁，体重指数25.7±8.0），分为A组——15名雌激素缺乏女性和B组——30名雌激素正常的对照组。所有女性均未绝经，也无高泌乳素血症。
在A组开始激素补充治疗前的标准条件下以及B组月经周期的第3 - 5天采集血样。采用标准放射免疫分析试剂盒测定血清hGH、IGF-I、IGFBP-3、胰岛素、睾酮、性激素结合球蛋白（SHBG）、硫酸脱氢表雄酮（DHEAS）和促黄体生成素（LH）以及泌乳素（PRL）、促卵泡生成素（FSH）和雌二醇水平。
与B组相比，A组的平均IGF-1、LH、FSH、睾酮、雌二醇和PRL血浆水平较低。SHBG、胰岛素和DHEAS的平均水平无显著差异。两组之间的平均年龄、体重、体重指数以及各体重指数范围的百分比也无差异。无论雌二醇水平如何，两组均发现了IGF-I与年龄的关联。两组均发现了IGF-I与IGFBP-3的关系。仅在A组中发现了IGF-I与雌二醇的关联。在B组中发现了hGH与SHBG的关联，在A组中这种关系是间接的。仅在B组中发现了hGH与睾酮水平的关联。在B组中，SHBG与IGFBP-3、睾酮和DHEAS相关。在B组中发现了胰岛素与IGFBP-3的关联。逐步多元回归显示，DHEAS和LH是A组中IGF-I水平的预测因子，而在B组中没有参数能够预测IGF-I水平。关于hGH的相同分析结果如下：在A组中，hGH水平由雌二醇和SHBG预测。在B组中，没有因素能够预测hGH水平。
血浆雌二醇水平与循环中的IGF-I相关，尽管这种关系似乎呈双相性。
