胃癌患者血清DNA甲基化与预后的相关性

Correlation between serum DNA methylation and prognosis in gastric cancer patients.

作者信息

Ikoma Hisashi, Ichikawa Daisuke, Koike Hiroshi, Ikoma Daito, Tani Nobuyuki, Okamoto Kazuma, Ochiai Toshiya, Ueda Yuji, Otsuji Eigo, Yamagishi Hisakazu

机构信息

Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.

出版信息

Anticancer Res. 2006 May-Jun;26(3B):2313-6.

PMID:16821608

Abstract

BACKGROUND

Gastric carcinogenesis is thought to involve multiple genetic and epigenetic changes. The relationships between the promoter methylation status of relevant genes in the serum and outcomes in patients undergoing curative gastrectomy for cancer were investigated.

MATERIALS AND METHODS

Pre-operative serum samples obtained from 97 gastric cancer patients, who underwent radical gastrectomy, were subjected to methylation-specific polymerase chain reaction (MSP) assays for the p16, E-cadherin and retinoic acid receptor beta (RARbeta) genes.

RESULTS

Promoter hypermethylation of p16, E-cadherin and the RARbeta gene was detected in sera from 18 (19%), 24 (25%) and 24 patients (25%), respectively. Altogether, 47 patients (48%) showed hypermethylation of at least one gene analyzed. Survival curves differed significantly between groups defined by the methylation status of E-cadherin (p<0.05), but not those defined by p16 or RARbeta (p =0. 77 and 0. 19, respectively).

CONCLUSION

Serum MSP assays can provide not only diagnostic, but also prognostic information in gastric cancer.

摘要

背景

胃癌发生被认为涉及多种基因和表观遗传学改变。本研究调查了血清中相关基因的启动子甲基化状态与接受胃癌根治性切除术患者预后之间的关系。

材料与方法

从97例行根治性胃切除术的胃癌患者术前采集血清样本，对p16、E-钙黏蛋白和视黄酸受体β（RARβ）基因进行甲基化特异性聚合酶链反应（MSP）检测。

结果

分别在18例（19%）、24例（25%）和24例（25%）患者的血清中检测到p16、E-钙黏蛋白和RARβ基因启动子高甲基化。共有47例（48%）患者至少有一个被分析基因发生高甲基化。根据E-钙黏蛋白甲基化状态定义的组间生存曲线有显著差异（p<0.05），而根据p16或RARβ定义的组间生存曲线无显著差异（分别为p = 0.77和0.19）。