Ichikawa Daisuke, Koike Hiroshi, Ikoma Hisashi, Ikoma Daito, Tani Nobuyuki, Otsuji Eigo, Kitamura Kazuya, Yamagishi Hisakazu
Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kawaramachihirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan.
Anticancer Res. 2004 Jul-Aug;24(4):2477-81.
This study aimed to detect hypermethylation in serum DNA from patients with gastric cancer at various stages and to assess the assay for early detection of primary and recurrent disease.
Preoperative serum samples were obtained from 109 patients with gastric cancer. Blood samples were subjected to methylation-specific polymerase chain reaction analysis to determine the methylation status of the promoter region of the p16 and E-cadherin genes.
Forty patients (37%) showed hypermethylation of the promoter region in one or both genes (p16, 20 patients; E-cadherin, 26 patients). Of 36 patients with early-stage gastric cancers, 10 (28%) showed aberrant methylated signals. No aberrant methylation was detected in the serum DNA from 10 healthy volunteers.
Aberrant promoter hypermethylation may prove useful as a new serum marker for gastric cancer.
本研究旨在检测不同阶段胃癌患者血清DNA中的高甲基化情况，并评估该检测方法用于早期检测原发性和复发性疾病的效果。
收集109例胃癌患者术前血清样本。对血样进行甲基化特异性聚合酶链反应分析，以确定p16和E-钙黏蛋白基因启动子区域的甲基化状态。
40例患者（37%）在一个或两个基因（p16，20例；E-钙黏蛋白，26例）的启动子区域出现高甲基化。36例早期胃癌患者中，10例（28%）出现异常甲基化信号。10名健康志愿者的血清DNA中未检测到异常甲基化。
启动子异常高甲基化可能作为一种新的胃癌血清标志物。
