Versari Silvia, Villa Anna Maria, Villa Alessandro, Doglia Silvia Maria, Pagani Giorgio A, Bradamante Silvia
CNR-ISTM, Institute of Molecular Science and Technology, Milan, Italy.
J Biomed Opt. 2006 May-Jun;11(3):34014. doi: 10.1117/1.2206173.
We propose a 2,5-Bis[1-(4-N-methylpyridinium)ethen-2-yl)]-N-methylpyrrole ditriflate (PEPEP) as a novel nontoxic, nonpotentiometric mitochondrial probe for confocal fluorescence microscopy. PEPEP is a representative chromophore of a large family of heterocyclic fluorescent dyes that show fluorescence emission in aqueous media and great DNA affinity. We check its cytotoxicity and intracellular localization in mammalian and yeast cell cultures. We demonstrate that PEPEP is a very efficient dye for fluorescence confocal microscopy and a valuable alternative to the most frequently used mitochondrial stains.
我们提出一种2,5-双[1-(4-N-甲基吡啶鎓)乙烯-2-基]-N-甲基吡咯二(三氟甲磺酸盐)(PEPEP)，作为一种用于共聚焦荧光显微镜的新型无毒、非电位型线粒体探针。PEPEP是一大类杂环荧光染料的代表性发色团，这类染料在水性介质中显示荧光发射且对DNA具有很强的亲和力。我们检测了它在哺乳动物和酵母细胞培养物中的细胞毒性和细胞内定位。我们证明PEPEP是一种用于荧光共聚焦显微镜的非常有效的染料，是最常用的线粒体染色剂的一种有价值的替代品。
