小鼠对脑膜炎球菌C多糖-蛋白结合物的黏膜免疫反应。

Mucosal immune responses to meningococcal C polysaccharide-protein conjugate in mice.

作者信息

Cuello Maribel, Cabrera Osmir, del Campo Judith M, Parajón Elina, Sotolongo Franklin, Camaraza Maria A, Arnet Aida, Suárez Magaly, Pérez Oliver

机构信息

Immunology Department, Finlay Institute, 27 Avenue No. 19805, P.O. Box 16017, Havana, Cuba.

出版信息

Vaccine. 2006 Apr 12;24 Suppl 2:S2-72-3. doi: 10.1016/j.vaccine.2005.01.129.

DOI:10.1016/j.vaccine.2005.01.129

PMID:16823934

Abstract

Mucosal delivery of vaccines represents an attractive approach because this is a region of first contact point for inhaled antigens. We have obtained a meningococcal group C polysaccharide-tetanus toxoid conjugate (MGCP-TT) and evaluated it for intranasal route in mice. The conjugate was obtained by a developed method in our laboratory. The specific IgA in saliva and specific IgA and IgG in serum were measured by ELISA methods and bactericidal antibodies in sera against a meningococcal group C strain were measured. The conjugated elicited a significant increase in anti-MGCP salivary IgA and serum IgG and bactericidal antibodies concentrations, while specific serum IgA was not observed. These results indicated that after conjugation, there was a change in the responses for MGCP from thymus-independent to thymus-dependent and that it was effective by intranasal route.

摘要

疫苗的黏膜给药是一种有吸引力的方法，因为这是吸入抗原的首个接触区域。我们获得了一种C群脑膜炎球菌多糖-破伤风类毒素结合物（MGCP-TT），并在小鼠中对其鼻内给药途径进行了评估。该结合物是通过我们实验室开发的方法获得的。采用酶联免疫吸附测定（ELISA）方法测定唾液中的特异性IgA以及血清中的特异性IgA和IgG，并测定血清中针对C群脑膜炎球菌菌株的杀菌抗体。该结合物引起抗MGCP唾液IgA、血清IgG和杀菌抗体浓度显著增加，而未观察到特异性血清IgA。这些结果表明，结合后，MGCP的反应从非胸腺依赖性转变为胸腺依赖性，并且鼻内给药途径有效。