Osawa Ro, Fujisawa Tomohiko, Pukall Rüdiger
Department of Bioresources and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, 657-8501, Japan.
Department of Food Science and Technology, Nippon Veterinary and Animal Science University, Kyonan-cho 1-7-1, Musashino-shi, Tokyo, 180-8602, Japan.
Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1693-1696. doi: 10.1099/ijs.0.64147-0.
A Gram-positive, rod-shaped, non-endospore-forming bacterium, strain ASB1(T), able to degrade tannin, was isolated from faeces of the Japanese large wood mouse, Apodemus speciosus. Comparative analysis of the 16S rRNA gene sequence revealed that the strain could be assigned as a member of the genus Lactobacillus. The nearest phylogenetic neighbours were determined as Lactobacillus animalis DSM 20602(T) (98.9 % 16S rRNA gene sequence similarity) and Lactobacillus murinus ASF 361 (98.9 %). Subsequent polyphasic analysis, including automated ribotyping and DNA-DNA hybridization experiments, confirmed that the isolate represents a novel species, for which the name Lactobacillus apodemi sp. nov. is proposed. The DNA G+C content of the novel strain is 38.5 mol%. The cell-wall peptidoglycan is of type A4alpha L-lys-D-asp. The type strain is ASB1(T) (=DSM 16634(T)=CIP 108913(T)).
从日本大林姬鼠(Apodemus speciosus)的粪便中分离出一株革兰氏阳性、杆状、不形成芽孢的细菌ASB1(T),该菌株能够降解单宁。16S rRNA基因序列的比较分析表明,该菌株可归为乳杆菌属的一个成员。其最近的系统发育邻种确定为动物乳杆菌DSM 20602(T)(16S rRNA基因序列相似性为98.9%)和鼠乳杆菌ASF 361(98.9%)。随后的多相分析,包括自动核糖体分型和DNA-DNA杂交实验,证实该分离物代表一个新物种,为此提出了新名称鼠乳杆菌(Lactobacillus apodemi sp. nov.)。该新菌株的DNA G+C含量为38.5 mol%。细胞壁肽聚糖为A4α型L-赖氨酸-D-天冬氨酸。模式菌株为ASB1(T)(=DSM 16634(T)=CIP 108913(T))。