Research Development Division, Maruzen Pharmaceuticals Co., Ltd., 14703-10 Mukaihigashi-cho, Onomichi 722-0062, Japan.
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokko-dai, Nada-ku, Kobe, 657-8501 Japan.
Biosci Microbiota Food Health. 2012;31(2):27-36. doi: 10.12938/bmfh.31.27. Epub 2012 Apr 20.
Complexing of green tea catechins with food constituents and their hydrolysis by tannase-producing Lactobacillus plantarum strains, were investigated. Our observations indicated that 1) epigallocatechin gallate (EGCg) and other catechin galloyl esters bound with food ingredients (i.e., proteins) to form a complex that is likely to be unabsorbable through the intestinal wall, whereas most catechins not esterified with gallic acid (GA) remain in free form, not complexing with food ingredients; 2) tannase activity of L. plantarum is strain dependent, possibly grouped into those with high tannase activity hydrolyzing EGCg to epigallocatechin and GA and those with the low activity; and 3) L. plantarum strains with high tannase activity are capable of hydrolyzing not only intact EGCg but also EGCg and other catechin galloyl esters complexed with dietary proteins to free non-galloyl ester catechins and GA. The evidence suggests that L. plantarum with high tannase activity, if it colonizes the human intestine, would release free non-galloyl-ester catechins and GA that are readily absorbed through the human intestinal epithelia from the complexes, thereby ensuring maximum delivery of the bioactive polyphenols of green tea to the host.
研究了绿茶儿茶素与食物成分的络合作用以及产单宁酶的植物乳杆菌菌株对其的水解作用。我们的观察结果表明：1）表没食子儿茶素没食子酸酯（EGCg）和其他儿茶素没食子酸酯与食物成分（如蛋白质）结合形成一种复合物，这种复合物可能无法穿过肠壁被吸收，而未与没食子酸酯化的大多数儿茶素仍以游离形式存在，不与食物成分结合；2）植物乳杆菌的单宁酶活性取决于菌株，可能分为高单宁酶活性菌株，其能将 EGCg 水解为表儿茶素和没食子酸，以及低活性菌株；3）高单宁酶活性的植物乳杆菌菌株不仅能够水解完整的 EGCg，还能够水解与膳食蛋白质结合的 EGCg 和其他儿茶素没食子酸酯，生成非没食子酰酯儿茶素和没食子酸。这些证据表明，如果高单宁酶活性的植物乳杆菌能够在人体肠道中定植，它将从复合物中释放出可被人体肠上皮细胞吸收的游离非没食子酰酯儿茶素和没食子酸，从而确保绿茶中生物活性多酚最大限度地被宿主吸收。
