Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
Brauerei C. & A. Veltins GmbH & Co. KG, An der Streue, 59872 Meschede-Grevenstein, Germany.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2353-2357. doi: 10.1099/ijs.0.018077-0. Epub 2009 Nov 20.
A Gram-stain-positive, catalase-negative and rod-shaped bacterium was isolated from a brewery environment. Its phylogenetic affiliation was determined by using 16S rRNA gene sequence analysis. It was found that strain TMW 1.1424(T) belongs to the genus Lactobacillus, with the three nearest neighbours Lactobacillus parabrevis LMG 11984(T) (97 %), Lactobacillus brevis DSM 20054(T) (95.9 %) and Lactobacillus hammesii DSM 16381(T) (96.2 %). Comparative sequencing of additional phylogenetic marker genes tuf and pheS confirmed the 16S rRNA gene sequence tree topology. The DNA G+C content of strain TMW 1.1424(T) is 46.6 mol%. Genomic DNA-DNA relatedness values with L. brevis DSM 20054(T), L. parabrevis LMG 11984(T) and L. hammesii DSM 16381(T) do not exceed 52.8 %, revealing that the novel isolate represents a separate genomic species. The strain can be distinguished from other related species of the genus Lactobacillus by physiological and biochemical tests. Based on biochemical, physiological and phylogenetic data, it is proposed that the new isolate be classified as a novel species of the genus Lactobacillus, Lactobacillus paucivorans sp. nov. The type strain is TMW 1.1424(T) (=DSM 22467(T) =LMG 25291(T)).
从啤酒厂环境中分离到一株革兰氏阳性、过氧化氢酶阴性的杆状细菌。通过 16S rRNA 基因序列分析确定其系统发育归属。结果表明，菌株 TMW 1.1424(T) 属于乳杆菌属，与其亲缘关系最近的三个种分别为短乳杆菌 LMG 11984(T)（97%）、短乳杆菌 DSM 20054(T)（95.9%）和哈氏乳杆菌 DSM 16381(T)（96.2%）。进一步测序分析了 tuf 和 pheS 这两个附加的系统发育标记基因，证实了 16S rRNA 基因序列树的拓扑结构。菌株 TMW 1.1424(T) 的 DNA G+C 含量为 46.6mol%。与 L. brevis DSM 20054(T)、L. parabrevis LMG 11984(T) 和 L. hammesii DSM 16381(T) 的基因组 DNA-DNA 同源性均不超过 52.8%，表明该新分离株代表了一个单独的基因组种。该菌株可通过生理生化试验与乳杆菌属的其他相关种相区别。基于生化、生理和系统发育数据，建议将新分离株分类为乳杆菌属的一个新种，即少孢乳杆菌（Lactobacillus paucivorans）sp. nov.，其模式菌株为 TMW 1.1424(T)（=DSM 22467(T) =LMG 25291(T)）。
