Langer-Wójcik Sabina, Czerwionka-Szaflarska Mieczysława
Katedra i Klinika Pediatrii, Alergologii i Gastroenterologii, Collegium Medicum, ul. M. Skolodowskiej - Curie 9, 85-094 Bydgoszcz, Poland.
Med Wieku Rozwoj. 2006 Apr-Jun;10(2):501-10.
during food provocation the mast cell degranulates causing the elevation of serum tryptase.
the aim of our work was to estimate if tryptase level is the efficient diagnostic marker in case of IgE dependent food allergy in children.
the studied group (group I) consisted of 100 children (age 3-5 yrs) with IgE dependent food allergy confirmed by positive case history and allergy tests. The children presented an allergic reaction to one of the 3 most common food allergens - milk, egg and wheat flour proteins. Control group (group II) consisted of 31 healthy children. The open food provocation was performed only in the group I. Tryptase level was estimated before and 120 minutes after the food provocation. The FluoroEnzymeImmunoAssay method (FEIA) was used to assess the tryptase levels.
baseline level of the tryptase in group I was significantly higher in comparison with group II. There was no significant difference between baseline level of the tryptase and level after the provocation. The decrease of the level of the tryptase after challenge was observed in 54% patients in group I. The increase of the tryptase level after the provocation was observed in 45% patients and in 1% patients the level of the tryptase was unchanged.
在食物激发试验期间，肥大细胞脱颗粒导致血清类胰蛋白酶升高。
我们研究的目的是评估类胰蛋白酶水平是否是儿童IgE介导的食物过敏的有效诊断标志物。
研究组（I组）由100名儿童（3 - 5岁）组成，这些儿童经阳性病史和过敏试验确诊为IgE介导的食物过敏。这些儿童对3种最常见的食物过敏原之一——牛奶、鸡蛋和小麦粉蛋白出现过敏反应。对照组（II组）由31名健康儿童组成。仅在I组进行开放性食物激发试验。在食物激发试验前和激发后120分钟评估类胰蛋白酶水平。采用荧光酶免疫分析法（FEIA）评估类胰蛋白酶水平。
I组类胰蛋白酶的基线水平与II组相比显著更高。类胰蛋白酶的基线水平与激发后水平之间无显著差异。I组中54%的患者在激发后类胰蛋白酶水平下降。45%的患者在激发后类胰蛋白酶水平升高，1%的患者类胰蛋白酶水平无变化。
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