Bony Sylvie, Carcelen Monique, Olivier Laurence, Devaux Alain
UMR INRA-DGER Mycotoxines et Toxicologie Comparée des Xénobiotiques, Ecole Nationale Vétérinaire de Lyon, F-69280 Marcy l'Etoile, France.
Toxicol Lett. 2006 Sep 30;166(1):67-76. doi: 10.1016/j.toxlet.2006.04.010. Epub 2006 Jun 3.
The genotoxic risk associated with deoxynivalenol (DON), a prevalent trichothecene mycotoxin which contaminates cereal-based products has not yet been deeply explored. In this work, the alkaline version of the Comet assay was used to evaluate DNA damage stemming from DON exposure in both dividing and differentiated Caco-2 cells, an epithelial intestinal cell line. To avoid false positive results, cytotoxic and apoptotic thresholds were firstly established using the MTS and neutral red assays and the Hoestch staining method, respectively. Dividing cells were found to be more sensitive to DON than differentiated cells and the lowest IC(10) (0.5 microM) obtained for dividing cells exposed for 72 h was used as the highest working concentration in the genotoxicity study. Both differentiated and dividing cells responded with a dose-dependent relationship to DON in terms of DNA damage in the 0.01-0.5 microM range. These results demonstrated the existence of a genotoxic potential for DON at low concentrations compatible with actual exposure situations and calls for additional studies to determine the functional consequences which could be taken into account for the risk assessment of this food contaminant.
脱氧雪腐镰刀菌烯醇（DON）是一种普遍存在的单端孢霉烯族霉菌毒素，会污染谷类产品，其相关的遗传毒性风险尚未得到深入研究。在这项研究中，采用彗星试验的碱性版本来评估DON暴露对上皮肠道细胞系Caco-2细胞在分裂期和分化期所造成的DNA损伤。为避免假阳性结果，首先分别使用MTS法、中性红试验和Hoestch染色法确定细胞毒性和凋亡阈值。研究发现，分裂期细胞比分化期细胞对DON更敏感，在遗传毒性研究中，将暴露72小时的分裂期细胞获得的最低IC(10)（0.5微摩尔）用作最高工作浓度。在0.01-0.5微摩尔范围内，分化期细胞和分裂期细胞的DNA损伤均与DON呈剂量依赖关系。这些结果表明，在与实际暴露情况相符的低浓度下，DON存在遗传毒性潜力，因此需要开展更多研究来确定其功能后果，以便在评估这种食品污染物的风险时加以考虑。
