iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal.
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Molecules. 2022 Jul 7;27(14):4356. doi: 10.3390/molecules27144356.
Natural deep eutectic solvents (NaDES) were used to extract bioactive compounds from marine by-products: codfish bones, mussel meat, and tuna vitreous humor. NaDES were prepared using natural compounds, including lactic acid (Lac), fructose (Fru), and urea (Ur), and were characterized to define their physicochemical properties, including the viscosity, density, surface tension, and refractive index. FTIR and NMR analysis confirmed the presence of intermolecular hydrogen bonding in NaDES. The extracts obtained using these NaDES were characterized to define their composition. Results demonstrated that the extract's composition differed highly, depending not only on the DES used, but also on the structure and composition of the raw material. Proteins and lipids were mainly present in extracts obtained from mussels, while ash content was highest in the extracts obtained from codfish bones. The biocompatibility of NaDES and the soluble fractions (SF) of the raw materials in NaDES was evaluated, and it was possible to conclude that the soluble ingredients obtained from the raw materials improved the biocompatibility of NaDES.
天然深共晶溶剂 (NaDES) 被用于从海洋副产物（鳕鱼骨、贻贝肉和金枪鱼玻璃体）中提取生物活性化合物。NaDES 是由天然化合物（包括乳酸 (Lac)、果糖 (Fru) 和尿素 (Ur)) 制备的，并对其理化性质进行了表征，包括粘度、密度、表面张力和折射率。FTIR 和 NMR 分析证实了 NaDES 中存在分子间氢键。使用这些 NaDES 获得的提取物的组成进行了定义。结果表明，提取物的组成差异很大，不仅取决于所使用的 DES，还取决于原料的结构和组成。蛋白质和脂质主要存在于从贻贝中提取的提取物中，而从鳕鱼骨中提取的提取物的灰分含量最高。评估了 NaDES 和 NaDES 中原料的可溶性部分 (SF) 的生物相容性，结果表明，从原料中获得的可溶性成分提高了 NaDES 的生物相容性。
