荧光团包裹的固体支撑双层囊泡：一种研究膜渗透过程的方法。

Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.

作者信息

Williams Thomas L, Vareiro Margarida M L M, Jenkins A Toby A

机构信息

Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.

出版信息

Langmuir. 2006 Jul 18;22(15):6473-6. doi: 10.1021/la060853q.

DOI:10.1021/la060853q

PMID:16830985

Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

摘要

这封信描述了一种研究膜裂解酶磷脂酶A(2)（PLA(2)）与磷脂双层相互作用的新方法，该方法通过使用表面等离子体共振（SPR）同时测量酶结合和囊泡裂解，并使用表面等离子体场增强荧光光谱（SPFS）测量通透性。将PLA(2)抑制剂二甲基二十碳二烯酸掺入表面结合的囊泡和支持双层中，以研究其在防止PLA(2)介导的囊泡裂解中的作用。这种方法对于研究一系列膜破坏剂具有普遍适用性。