用人血清对单纯疱疹病毒多肽进行免疫沉淀与其酶联免疫吸附测定（ELISA）滴度相关。
Immunoprecipitation of herpes simplex virus polypeptides with human sera is related to their ELISA titre.
作者信息
Murányiová M, Rajcáni J, Krivjanská M, Matis J, Pogády J
机构信息
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czecho-Slovakia.
出版信息
Acta Virol. 1991 May;35(3):252-9.
Out of 485 human sera tested by neutralization and enzyme immunoassay 13 were negative in both tests, while a positive correlation was found in 457 samples (94.3%). The rest of sera (3.2%) showed discordant results. Selected sera were examined, in addition, by Western blot and immunoprecipitation assays with the aim to analyse the precipitation profile of discordant sera and correlate the ELISA titre with the precipitation profiles. High titre sera precipitated the main glycoproteins (gC, gB, gE, gD), the capsid polypeptides (VP 5, VP 19, VP 21, VP 22) and several other structural and nonstructural proteins. The ability of sera to precipitate viral structural proteins was related to their ELISA titre rather than to their neutralizing activity.
在通过中和试验和酶免疫测定法检测的485份人血清中，13份在两种检测中均为阴性，而在457份样本（94.3%）中发现呈正相关。其余血清（3.2%）结果不一致。此外，对选定的血清进行了蛋白质印迹法和免疫沉淀试验，目的是分析结果不一致血清的沉淀图谱，并将酶联免疫吸附测定（ELISA）滴度与沉淀图谱相关联。高滴度血清沉淀出主要糖蛋白（gC、gB、gE、gD）、衣壳多肽（VP 5、VP 19、VP 21、VP 22）以及其他几种结构和非结构蛋白。血清沉淀病毒结构蛋白的能力与其ELISA滴度有关，而与其中和活性无关。
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