Liu Qian, Liu Yingju, Li Yangqing, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China.
J Sep Sci. 2006 Jun;29(9):1268-74. doi: 10.1002/jssc.200600032.
LIF detection is one of the most sensitive detection methods for CE. However, its application is limited because the analyte is usually required to be derivatized with a fluorescent label. As a result, LIF is seldom used to analyze active ingredients in plants. In this work, we introduce a rapid, simple, and sensitive method of nonaqueous CE (NACE) coupled with laser-induced native fluorescence detection for the simultaneous analysis of berberine, palmatine, and jatrorrhizine. This method skillfully utilizes the native fluorescence of these alkaloids and requires no troublesome fluorescent derivatization. As these alkaloids can fluoresce to some degree, they were simply detected by a commercially available 488 nm Ar+ laser. The native fluorescence of the analytes was greatly enhanced by nonaqueous media. Compared with the reported UV detection method, much lower LOD was achieved (6.0 ng/mL for berberine, 7.5 ng/mL for palmatine, and 380 ng/mL for jatrorrhizine). This method was successfully applied to analyze berberine, palmatine, and jatrorrhizine in two Chinese herbal medicines, Rhizoma coptidis and Caulis mahoniae.
激光诱导荧光检测是毛细管电泳中最灵敏的检测方法之一。然而，由于通常需要用荧光标记物对分析物进行衍生化处理，其应用受到限制。因此，激光诱导荧光很少用于分析植物中的活性成分。在本研究中，我们介绍了一种快速、简便且灵敏的非水毛细管电泳（NACE）结合激光诱导天然荧光检测的方法，用于同时分析小檗碱、巴马汀和药根碱。该方法巧妙地利用了这些生物碱的天然荧光，无需繁琐的荧光衍生化。由于这些生物碱能在一定程度上发出荧光，只需用市售的488 nm氩离子激光进行简单检测。非水介质极大地增强了分析物的天然荧光。与报道的紫外检测方法相比，检测限更低（小檗碱为6.0 ng/mL，巴马汀为7.5 ng/mL，药根碱为380 ng/mL）。该方法成功应用于分析两种中药材黄连和十大功劳茎中的小檗碱、巴马汀和药根碱。
