[蒸馏水在精子计数及低渗肿胀试验中的应用]
[Application of distilled water in sperm counting and hypoosmotic swelling test].
作者信息
Chen Fang, Lu Jin-chun, Xu Hui-ru, Huang Yu-feng, Hu Yu-an, Shao Yong
机构信息
Clinical School, Nanjing University Medical College, Department of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
出版信息
Zhonghua Nan Ke Xue. 2006 Jun;12(6):542-4.
OBJECTIVE
To evaluate the application of distilled water in sperm-counting and hypoosmotic swelling test.
METHODS
Thirty-seven semen samples were collected and each was diluted by distilled water and sodium acid carbonate-formaldehyde solution, respectively. Then the hemacytometer was used for sperm counting. Meanwhile, the percentage of swelled sperm diluted by distilled water was compared with the result of hypoosmotic swelling test recommended by WHO. Another 26 semen samples were diluted by distilled water and hypoosmotic swelling solution respectively, and the percentages of the swelled sperm were compared.
RESULTS
There was no significant difference either between the sperm concentrations obtained by distilled water and sodium acid carbonate-formaldehyde solution (P > 0.05) or between the percentages of the swelled sperm diluted by distilled water and hypoosmotic swelling solution.
CONCLUSION
Distilled water can not only replace sodium acid carbonate-formaldehyde solution for sperm-counting dilution but also be used as a hypoosmotic swelling solution.
目的
评估蒸馏水在精子计数和低渗肿胀试验中的应用。
方法
收集37份精液样本，每份样本分别用蒸馏水和碳酸氢钠 - 甲醛溶液进行稀释。然后使用血细胞计数器进行精子计数。同时，将用蒸馏水稀释的肿胀精子百分比与世界卫生组织推荐的低渗肿胀试验结果进行比较。另外26份精液样本分别用蒸馏水和低渗肿胀溶液进行稀释，并比较肿胀精子的百分比。
结果
蒸馏水和碳酸氢钠 - 甲醛溶液获得的精子浓度之间（P>0.05）以及蒸馏水和低渗肿胀溶液稀释的肿胀精子百分比之间均无显著差异。
结论
蒸馏水不仅可以替代碳酸氢钠 - 甲醛溶液用于精子计数稀释，还可作为低渗肿胀溶液使用。
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