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低渗肿胀“水试验”的可用性——一种评估小鼠精子细胞膜完整性的简单方法。

Usability of the hypoosmotic swelling "Water-test"--a simple method to assess sperm membrane integrity in mouse spermatozoa.

作者信息

Sliwa L

机构信息

Department of Embryology and Medical Genetics, Collegium Medicum, Jagiellonian University, Kraków, Poland.

出版信息

Folia Biol (Krakow). 1993;41(1-2):29-31.

PMID:8137993
Abstract

The functional integrity of the membrane of sperm from 3 strains of mice (BALB/c, CBA/KW, and C57BL/KW) was assessed by studying the swelling reaction of the sperm when suspended in a medium of distilled water. The results were correlated with analysis of sperm motility and those of Eosin-Y staining. The results obtained from the Eosin-Y test and "Water-test" differed (P < 0.01) between strains C57BL/KW and both BALB/C, and CBA/KW. A good correlation was observed in all strains of mice between the values of the "Water-test" and those for Eosin-Y staining (P < 0.01).

摘要

通过研究精子悬浮于蒸馏水介质中的肿胀反应，评估了3种品系小鼠（BALB/c、CBA/KW和C57BL/KW）精子膜的功能完整性。将结果与精子活力分析以及伊红-Y染色结果相关联。C57BL/KW品系与BALB/C和CBA/KW品系之间，伊红-Y试验和“水试验”的结果存在差异（P<0.01）。在所有品系小鼠中，“水试验”值与伊红-Y染色值之间均观察到良好的相关性（P<0.01）。

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