Fuse H, Ohta S, Sakamoto M, Kazama T, Katayama T
Department of Urology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Arch Androl. 1993 Mar-Apr;30(2):111-6. doi: 10.3109/01485019308987743.
The functional competence of the human sperm membrane is assessed by studying the swelling reaction of sperm when suspended in a medium of distilled water (water test). Eighty-seven patients with idiopathic infertility were investigated by the water test and the results were compared with various semen parameters. High correlations with the percentage of b-g type swollen sperm in the water test were observed for sperm concentration (r = .53, p < .05) and percent motility (r = .62, p < .01). The sperm swelling values obtained by the water test correlated well with those obtained by the hypoosmotic swelling test (HOST). Correlation coefficients in b-g and g swelling pattern were 0.89 and 0.71, respectively. The percentage of g-type swelling obtained by the water test was significantly larger than that obtained by the HOST (p < .05), although the two hypoosmotic procedures gave similar percentages of b-g swelling pattern. The percentage of g swelling pattern obtained by the water test correlated well with percent motility (r = .70, p < .001). These findings suggest that the water test is more useful for assessing the integrity of the sperm membrane than the HOST. The relationship between this test and subsequent fertility, as well as to other sperm function tests, needs further investigation.
通过研究精子悬浮于蒸馏水介质中的肿胀反应（水试验）来评估人类精子膜的功能能力。对87例特发性不育患者进行了水试验，并将结果与各种精液参数进行比较。水试验中b-g型肿胀精子的百分比与精子浓度（r = 0.53，p < 0.05）和活动率百分比（r = 0.62，p < 0.01）高度相关。水试验获得的精子肿胀值与低渗肿胀试验（HOST）获得的值相关性良好。b-g和g肿胀模式的相关系数分别为0.89和0.71。尽管两种低渗方法得到的b-g肿胀模式百分比相似，但水试验获得的g型肿胀百分比显著高于HOST获得的百分比（p < 0.05）。水试验获得的g肿胀模式百分比与活动率百分比相关性良好（r = 0.70，p < 0.001）。这些发现表明，与HOST相比，水试验在评估精子膜完整性方面更有用。该试验与后续生育能力以及其他精子功能试验之间的关系需要进一步研究。
