Lee Silvia, Watson Mark W, Clark Ben, Flexman James P, Cheng Wendy, French Martyn A H, Price Patricia
Department of Clinical Immunology and Biochemical Genetics, Royal Perth Hospital, Perth, Western Australia, Australia.
Immunol Cell Biol. 2006 Aug;84(4):390-5. doi: 10.1111/j.1440-1711.2006.01451.x.
Rapid progression of hepatitis C virus (HCV) disease in patients with HIV/HCV may reflect different cytokine responses and be influenced by HCV genotype. This is addressed by a study of patients with HIV/HCV coinfection and infection with HCV genotype 2 or 3 (2/3). They are compared with coinfected patients infected with genotype 1 and HCV monoinfected patients matched for HCV genotype. IFN-gamma, IL-10, IL-4 and IL-4delta2 mRNA were quantified by real-time PCR in unstimulated PBMC and after in vitro stimulation with HCV core or nonstructural 3/4A antigen. In unstimulated PBMC, levels of IFN-gamma and IL-4 mRNA were lowest in HIV/HCV genotype 1 patients, intermediate in HIV/HCV genotype 2/3 patients and highest in HCV genotype 2/3 patients. Neither HCV genotype nor HIV affected levels of IL-10 mRNA in unstimulated PBMC or IFN-gamma, IL-4 and IL-10 mRNA in PBMC stimulated with HCV antigens. Levels of IL-4 and IL-4delta2 mRNA correlated in mitogen-stimulated PBMC from all patient groups but both were low in HIV/HCV genotype 1 patients. Serum soluble CD30 levels (a putative marker of a T2 cytokine environment) did not differ between patient groups. The data do not suggest a shift in the T1/T2 balance driven by HIV coinfection or HCV genotype but either may affect IL-4 bioavailability.
人类免疫缺陷病毒（HIV）/丙型肝炎病毒（HCV）合并感染患者中丙型肝炎病毒（HCV）疾病的快速进展可能反映出不同的细胞因子反应，并受HCV基因型影响。一项针对HIV/HCV合并感染以及感染HCV基因2型或3型（2/3型）患者的研究探讨了这一问题。将他们与感染基因1型的合并感染患者以及按HCV基因型匹配的HCV单一感染患者进行比较。通过实时聚合酶链反应（PCR）对未受刺激的外周血单个核细胞（PBMC）以及经HCV核心抗原或非结构蛋白3/4A抗原体外刺激后的PBMC中的γ干扰素（IFN-γ）、白细胞介素10（IL-10）、白细胞介素4（IL-4）和IL-4δ2信使核糖核酸（mRNA）进行定量分析。在未受刺激的PBMC中，HIV/HCV基因1型患者的IFN-γ和IL-4 mRNA水平最低，HIV/HCV基因2/3型患者的水平处于中间，而HCV基因2/3型患者的水平最高。HCV基因型和HIV均未影响未受刺激的PBMC中IL-10 mRNA的水平，也未影响经HCV抗原刺激后的PBMC中IFN-γ、IL-4和IL-10 mRNA的水平。在所有患者组经丝裂原刺激的PBMC中，IL-4和IL-4δ2 mRNA水平呈正相关，但在HIV/HCV基因1型患者中两者均较低。各患者组之间血清可溶性CD30水平（T2细胞因子环境的一种假定标志物）并无差异。数据并不表明由HIV合并感染或HCV基因型驱动的T1/T2平衡发生改变，但两者均可能影响IL-4的生物利用度。
