Kanamaru Sojun, Kurazono Hisao, Nakano Masayuki, Terai Akito, Ogawa Osamu, Yamamoto Shingo
Department of Urology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Int J Urol. 2006 Jun;13(6):754-60. doi: 10.1111/j.1442-2042.2006.01398.x.
Phylogenetic analysis has been used widely to characterize extraintestinal pathogenic Escherichia coli in molecular epidemiological studies. We have recently reported a putative pathogenicity island (PAI), carrying uropathogenic-specific protein (usp) and a unique mosaic structure of small open reading frames following usp, providing four subtypes of PAIusp classified from their sequential patterns.
A total of 427 E. coli isolates from uncomplicated urinary tract infections (194 cystitis, 76 pyelonephritis, and 107 prostatitis) and 50 fecal isolates were examined for phylogenetic grouping and PAIusp subtyping as well as the prevalence of virulence factors (VF) and O serogroups.
Both phylogenetic group B2 and usp-positive strains were equally predominant in cystitis, pyelonephritis and prostatitis (B2, 80.9%, 86.8%, and 86.9%; usp, 79.4%, 93.4%, and 88.8%, respectively). Furthermore, each PAIusp subtype was shown to be closely associated with several VF genes as well as several common O serogroups of uropathogenic E. coli.
In molecular epidemiological studies, PAIusp subtyping will provide additional informative findings of E. coli strains belonging to phylogenetic group B2.
在分子流行病学研究中，系统发育分析已被广泛用于鉴定肠道外致病性大肠杆菌。我们最近报道了一个假定的致病岛（PAI），其携带泌尿道致病性特异性蛋白（usp）以及usp之后小开放阅读框的独特镶嵌结构，根据其序列模式可将PAIusp分为四种亚型。
对427株来自单纯性尿路感染（194例膀胱炎、76例肾盂肾炎和107例前列腺炎）的大肠杆菌分离株以及50株粪便分离株进行系统发育分组和PAIusp亚型分析，同时检测毒力因子（VF）和O血清群的流行情况。
系统发育组B2和usp阳性菌株在膀胱炎、肾盂肾炎和前列腺炎中同样占主导地位（B2分别为80.9%、86.8%和86.9%；usp分别为79.4%、93.4%和88.8%）。此外，每种PAIusp亚型均显示与几种VF基因以及几种常见的泌尿道致病性大肠杆菌O血清群密切相关。
在分子流行病学研究中，PAIusp亚型分析将为属于系统发育组B2的大肠杆菌菌株提供更多有价值的发现。
