Lédée Nathalie, Dubanchet Sylvie, Lombroso Raoul, Ville Yves, Chaouat Gérard
Université Versailles- St Quentin en Yvelines, Centre Hospitalier de Poissy- St Germain en Laye, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, France.
Am J Reprod Immunol. 2006 Aug;56(2):119-23. doi: 10.1111/j.1600-0897.2006.00401.x.
To evaluate the influence of ovarian steroids on IL-18, IL-15 and angiopoietin-2 mRNA expression in the endometrium in the mid luteal phase.
We quantified IL-18/GAPDH, IL-18 BP/GAPDH, IL-15/GAPDH and angiopoietin-2/GAPDH in the endometrium by quantitative polymerase chain reaction on day 21 of the cycle. We first compared cytokines expression over two natural cycles (n = 15) then between natural and oestrogen-progestin replacement treatment (n = 18).
Endometrial IL-18, IL-18 BP, IL-15 and angiopoietin-2 mRNA expression did not change over two natural cycles. Addition of exogenous hormones significantly decreased IL-18 and IL-18 BP mRNA expression but not influence IL-15 or angiopoietin-2 ratios. This was also observed with immunohistochemistry.
Exogenous oestro-progestative hormones influence endometrial IL-18 system expression involved in angiogenesis and in the uterine natural killer (uNK) cell activation pathway during the implantation process.
评估卵巢类固醇对黄体中期子宫内膜中白细胞介素-18（IL-18）、白细胞介素-15（IL-15）和血管生成素-2（angiopoietin-2）mRNA表达的影响。
在月经周期的第21天，通过定量聚合酶链反应对子宫内膜中的IL-18/甘油醛-3-磷酸脱氢酶（GAPDH）、IL-18结合蛋白（IL-18 BP）/GAPDH、IL-15/GAPDH和血管生成素-2/GAPDH进行定量分析。我们首先比较了两个自然周期（n = 15）中细胞因子的表达情况，然后比较了自然周期与雌激素-孕激素替代治疗（n = 18）之间细胞因子的表达情况。
在两个自然周期中，子宫内膜的IL-18、IL-18 BP、IL-15和血管生成素-2 mRNA表达没有变化。添加外源性激素显著降低了IL-18和IL-18 BP mRNA的表达，但不影响IL-15或血管生成素-2的比例。免疫组织化学分析也观察到了这一现象。
外源性雌激素-孕激素会影响子宫内膜IL-18系统的表达，该系统在植入过程中参与血管生成和子宫自然杀伤（uNK）细胞激活途径。
