Kim Sang-Soo, Lim Sang-Hyun, Hong Yoo-Sun, Cho Seung-Woo, Ryu Ju Hee, Chang Byung-Chul, Choi Cha Yong, Kim Byung-Soo
Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Korea.
Artif Organs. 2006 Jul;30(7):554-7. doi: 10.1111/j.1525-1594.2006.00258.x.
In this study, we tissue-engineered heart valves in vivo using autologous bone marrow-derived cells (BMCs). Canine BMCs were differentiated into endothelial cell (EC)-like cells and myofibroblast (MF)-like cells. Decellularized porcine pulmonary valves were seeded with BMCs and implanted to abdominal aorta and pulmonary valve of bone marrow donor dogs. Histological examination of the explants identified the regeneration of valvular structures expressing CD31 and smooth muscle alpha-actin, indicating the presence of EC-like and MF-like cells in the grafts at 3 and 1 week, respectively, after implantation. Fluorescent microscopic examinations identified the presence of fluorescently labeled cells in the explants, indicating that the implanted BMCs survived and participated in the heart valve reconstitution. This study reports, for the first time, on tissue engineering of heart valve in vivo using BMCs.
在本研究中，我们使用自体骨髓来源细胞（BMCs）在体内构建组织工程心脏瓣膜。犬BMCs分化为内皮细胞（EC）样细胞和成肌纤维细胞（MF）样细胞。将脱细胞猪肺动脉瓣接种BMCs后植入骨髓供体犬的腹主动脉和肺动脉瓣。对取出的移植物进行组织学检查，发现表达CD31和平滑肌α-肌动蛋白的瓣膜结构再生，分别表明植入后3周和1周时移植物中存在EC样细胞和MF样细胞。荧光显微镜检查发现取出的移植物中存在荧光标记细胞，表明植入的BMCs存活并参与了心脏瓣膜重构。本研究首次报道了使用BMCs在体内进行心脏瓣膜组织工程的研究。
