Ward T H, Brandizzi F
Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
Cell Mol Life Sci. 2004 Jan;61(2):172-85. doi: 10.1007/s00018-003-3355-6.
In less than a decade the green fluorescent protein (GFP) has become one of the most popular tools for cell biologists for the study of dynamic processes in vivo. GFP has revolutionised the scientific approach for the study of vital organelles, such as the Golgi apparatus. As Golgi proteins can be tagged with GFP, in most cases without altering their targeting and function, it is a great substitute to conventional dyes used in the past to highlight this compartment. In this review, we cover the application of GFP and its spectral derivatives in the study of Golgi dynamics in mammalian and plant cells. In particular, we focus on the technique of selective photobleaching known as fluorescence recovery after photobleaching, which has successfully shed light on essential differences in the biology of the Golgi apparatus in mammalian and plant cells.
在不到十年的时间里，绿色荧光蛋白（GFP）已成为细胞生物学家研究体内动态过程最常用的工具之一。GFP彻底改变了研究重要细胞器（如高尔基体）的科学方法。由于高尔基体蛋白可以用GFP标记，在大多数情况下不会改变其靶向性和功能，它是过去用于突出该细胞器的传统染料的理想替代品。在这篇综述中，我们阐述了GFP及其光谱衍生物在哺乳动物和植物细胞高尔基体动力学研究中的应用。特别是，我们重点关注了选择性光漂白技术，即光漂白后荧光恢复，它成功地揭示了哺乳动物和植物细胞中高尔基体生物学的本质差异。
