高尔基体膜中蛋白质的动态变化：光漂白技术突显的哺乳动物细胞与植物细胞之间的比较
Dynamics of proteins in Golgi membranes: comparisons between mammalian and plant cells highlighted by photobleaching techniques.
作者信息
Ward T H, Brandizzi F
机构信息
Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
出版信息
Cell Mol Life Sci. 2004 Jan;61(2):172-85. doi: 10.1007/s00018-003-3355-6.
Abstract
In less than a decade the green fluorescent protein (GFP) has become one of the most popular tools for cell biologists for the study of dynamic processes in vivo. GFP has revolutionised the scientific approach for the study of vital organelles, such as the Golgi apparatus. As Golgi proteins can be tagged with GFP, in most cases without altering their targeting and function, it is a great substitute to conventional dyes used in the past to highlight this compartment. In this review, we cover the application of GFP and its spectral derivatives in the study of Golgi dynamics in mammalian and plant cells. In particular, we focus on the technique of selective photobleaching known as fluorescence recovery after photobleaching, which has successfully shed light on essential differences in the biology of the Golgi apparatus in mammalian and plant cells.
摘要
在不到十年的时间里，绿色荧光蛋白（GFP）已成为细胞生物学家研究体内动态过程最常用的工具之一。GFP彻底改变了研究重要细胞器（如高尔基体）的科学方法。由于高尔基体蛋白可以用GFP标记，在大多数情况下不会改变其靶向性和功能，它是过去用于突出该细胞器的传统染料的理想替代品。在这篇综述中，我们阐述了GFP及其光谱衍生物在哺乳动物和植物细胞高尔基体动力学研究中的应用。特别是，我们重点关注了选择性光漂白技术，即光漂白后荧光恢复，它成功地揭示了哺乳动物和植物细胞中高尔基体生物学的本质差异。
相似文献
1
Dynamics of proteins in Golgi membranes: comparisons between mammalian and plant cells highlighted by photobleaching techniques.高尔基体膜中蛋白质的动态变化：光漂白技术突显的哺乳动物细胞与植物细胞之间的比较
Cell Mol Life Sci. 2004 Jan;61(2):172-85. doi: 10.1007/s00018-003-3355-6.
2
Photobleaching methods to study Golgi complex dynamics in living cells.用于研究活细胞中高尔基体复合体动力学的光漂白方法。
Methods Cell Biol. 2013;118:195-216. doi: 10.1016/B978-0-12-417164-0.00012-4.
3
Organelle motility in plant cells: imaging golgi and ER dynamics with GFP.植物细胞中的细胞器运动：利用绿色荧光蛋白成像高尔基体和内质网动态
Curr Protoc Cell Biol. 2001 May;Chapter 13:Unit 13.3. doi: 10.1002/0471143030.cb1303s09.
4
Photobleaching approaches to investigate diffusional mobility and trafficking of Ras in living cells.利用光漂白方法研究活细胞中Ras的扩散迁移率和运输情况。
Methods. 2005 Oct;37(2):154-64. doi: 10.1016/j.ymeth.2005.05.013.
5
On and off membrane dynamics of the endoplasmic reticulum-golgi tethering factor p115 in vivo.内质网-高尔基体拴系因子p115在体内的膜内外动态变化
Mol Biol Cell. 2006 Jul;17(7):2996-3008. doi: 10.1091/mbc.e05-09-0862. Epub 2006 Apr 19.
6
Imaging the Golgi apparatus in living mitotic cells.对有丝分裂活细胞中的高尔基体进行成像。
Methods Mol Biol. 2007;390:309-28. doi: 10.1007/978-1-59745-466-7_21.
7
The plant Golgi apparatus.植物高尔基体
Biochim Biophys Acta. 1998 Aug 14;1404(1-2):259-70. doi: 10.1016/s0167-4889(98)00061-5.
8
Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network.轨道上的堆栈：植物高尔基体在肌动蛋白/内质网网络上运输。
Plant J. 1998 Aug;15(3):441-7. doi: 10.1046/j.1365-313x.1998.00208.x.
9
Monitoring and quantifying dynamic physiological processes in live neurons using fluorescence recovery after photobleaching.利用光漂白后荧光恢复监测和量化活神经元中的动态生理过程。
J Neurochem. 2013 Jul;126(2):213-22. doi: 10.1111/jnc.12240. Epub 2013 Apr 16.
10
Plant organelle positioning.植物细胞器定位
Curr Opin Plant Biol. 2004 Dec;7(6):626-31. doi: 10.1016/j.pbi.2004.09.005.
引用本文的文献
1
Trafficking and localization of Golgi-resident -glycan processing enzymes in plants.植物中高尔基体驻留聚糖加工酶的运输与定位
Front Plant Sci. 2025 Jul 25;16:1624949. doi: 10.3389/fpls.2025.1624949. eCollection 2025.
2
Three-dimensional analysis of membrane structures associated with tomato spotted wilt virus infection.与番茄斑萎病毒感染相关的膜结构的三维分析。
Plant Cell Environ. 2023 Feb;46(2):650-664. doi: 10.1111/pce.14511. Epub 2022 Dec 19.
3
Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner.KCNJ3基因剪接变体的过表达以相反的方式影响恶性乳腺癌细胞系MCF-7的重要参数。
BMC Cancer. 2016 Aug 12;16:628. doi: 10.1186/s12885-016-2664-8.
4
ER - the key to the highway.内质网——通向高速公路的关键。
Curr Opin Plant Biol. 2014 Dec;22:30-38. doi: 10.1016/j.pbi.2014.09.001. Epub 2014 Sep 26.
5
ARF1 and SAR1 GTPases in endomembrane trafficking in plants.植物细胞内膜运输中的 ARF1 和 SAR1 GTPases。
Int J Mol Sci. 2013 Sep 5;14(9):18181-99. doi: 10.3390/ijms140918181.
6
Organization of the ER-Golgi interface for membrane traffic control.内质网-高尔基体界面的组织对于膜运输控制至关重要。
Nat Rev Mol Cell Biol. 2013 Jun;14(6):382-92. doi: 10.1038/nrm3588.
7
Four and a half LIM protein 1C (FHL1C): a binding partner for voltage-gated potassium channel K(v1.5).四半 LIM 蛋白 1C（FHL1C）：电压门控钾通道 K（v1.5）的结合伴侣。
PLoS One. 2011;6(10):e26524. doi: 10.1371/journal.pone.0026524. Epub 2011 Oct 28.
8
Sub-compartmental organization of Golgi-resident N-glycan processing enzymes in plants.植物中高尔基体驻留 N-聚糖加工酶的亚区室组织。
Mol Plant. 2011 Mar;4(2):220-8. doi: 10.1093/mp/ssq082. Epub 2011 Feb 9.
9
Sequential depletion and acquisition of proteins during Golgi stack disassembly and reformation.在高尔基堆栈解体和重建过程中蛋白质的顺序耗竭和获得。
Traffic. 2010 Nov;11(11):1429-44. doi: 10.1111/j.1600-0854.2010.01106.x. Epub 2010 Aug 18.
10
New gateways to discovery.新的发现途径。
Plant Physiol. 2007 Dec;145(4):1100-9. doi: 10.1104/pp.107.106641.
本文引用的文献
1
Factors affecting residence time of mesenchymal stromal cells (MSC) injected into the myocardium.影响注射到心肌中的间充质基质细胞 (MSC) 停留时间的因素。
Cell Transplant. 2010;19(8):937-48. doi: 10.3727/096368910X494911. Epub 2010 Mar 26.
2
Early failure of xenogenous de-cellularised pulmonary valve conduits--a word of caution!异种去细胞化肺动脉带瓣管道早期失败——警告！
Eur J Cardiothorac Surg. 2010 Jul;38(1):78-85. doi: 10.1016/j.ejcts.2010.01.044. Epub 2010 Mar 12.
3
Tissue engineering and regenerative medicine: from first principles to state of the art.组织工程与再生医学：从基本原理到最新进展。
J Pediatr Surg. 2010 Feb;45(2):291-4. doi: 10.1016/j.jpedsurg.2009.10.063.
4
Late outcomes following freestyle versus homograft aortic root replacement: results from a prospective randomized trial.游离主动脉瓣环置换术与同种异体主动脉瓣环置换术的远期结果：前瞻性随机试验结果。
J Am Coll Cardiol. 2010 Jan 26;55(4):368-76. doi: 10.1016/j.jacc.2009.09.030.
5
Late-term results of tissue-engineered vascular grafts in humans.人体组织工程血管移植物的晚期结果。
J Thorac Cardiovasc Surg. 2010 Feb;139(2):431-6, 436.e1-2. doi: 10.1016/j.jtcvs.2009.09.057.
6
Tissue-engineered heart valve scaffolds.组织工程心脏瓣膜支架
Ann Thorac Cardiovasc Surg. 2009 Dec;15(6):362-7.
7
From stem cells and cadaveric matrix to engineered organs.从干细胞和尸体基质到工程器官。
Curr Opin Biotechnol. 2009 Oct;20(5):598-605. doi: 10.1016/j.copbio.2009.10.016. Epub 2009 Nov 13.
8
Mesenchymal stromal cells for cardiovascular repair: current status and future challenges.用于心血管修复的间充质基质细胞：现状与未来挑战
Future Cardiol. 2009 Nov;5(6):605-17. doi: 10.2217/fca.09.42.
9
AN EXPERIMENTAL INVESTIGATION OF THE TREATMENT OF WOUNDS OF THE HEART BY MEANS OF SUTURE OF THE HEART MUSCLE.心肌缝合治疗心脏创伤的实验研究。
J Exp Med. 1899 Sep 1;4(5-6):479-520. doi: 10.1084/jem.4.5-6.479.
10
Cardiomyocyte differentiation of human induced pluripotent stem cells.人诱导多能干细胞的心肌细胞分化
Circulation. 2009 Oct 13;120(15):1513-23. doi: 10.1161/CIRCULATIONAHA.109.868885. Epub 2009 Sep 28.
Zotero 插件