Reynaud Stéphane, Malissein Emilie, Donnard Magali, Bordessoule Dominique, Turlure Pascal, Trimoreau Franck, Denizot Yves
UMR CNRS 6101, Centre National de la Recherche Scientifique, Université de Limoges, France.
Leuk Res. 2007 Mar;31(3):399-402. doi: 10.1016/j.leukres.2006.06.002. Epub 2006 Jul 11.
Platelet-activating factor (PAF) is a phospholipid mediator with potent immunoregulatory activities on mature leukocytes. PAF modulates leukocyte cytosolic Ca2+ concentration ([Ca2+]i) through a Gq mediated pathway. We highlight, for the first time, Gq transcripts, PAF receptor (PAF-R) transcripts and protein in blast cells of acute myeloid (AML) and lymphoid (ALL) leukemia patients. PAF stimulated [Ca2+]i in leukemic blast cells; PAF effects being prevented by a specific PAF-R antagonist. In conclusion, functional PAF-R are present in blast cells of patients with acute leukemia; a result that could be of physiologic importance regarding the important effect of PAF on leukocytes maturation and functions.
血小板活化因子（PAF）是一种对成熟白细胞具有强大免疫调节活性的磷脂介质。PAF通过Gq介导的途径调节白细胞胞质Ca2+浓度（[Ca2+]i）。我们首次在急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）患者的原始细胞中发现了Gq转录本、PAF受体（PAF-R）转录本和蛋白。PAF刺激白血病原始细胞中的[Ca2+]i；PAF的作用可被特异性PAF-R拮抗剂阻断。总之，急性白血病患者的原始细胞中存在功能性PAF-R；这一结果对于PAF对白细胞成熟和功能的重要影响可能具有生理意义。
