Singh G, Kaur S, Stock J L, Jenkins N A, Gilbert D J, Copeland N G, Potter S S
Department of Basic Sciences, Children's Hospital Medical Center, Cincinnati, OH 45229.
Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10706-10. doi: 10.1073/pnas.88.23.10706.
In Drosophila a number of genes important in establishing segmentation patterns and in determining segment identities have been shown to carry the homeobox sequence. Over 30 murine homeobox genes have been cloned, many on the basis of sequence homology to Drosophila prototypes. Here we report the cloning and sequencing of 10 new and 6 previously known homeobox genes by screening a murine genomic library with a 768-fold degenerate oligonucleotide corresponding to the most conserved 8-amino acid motif in the recognition helix of the homeodomain. Eight of these new homeobox genes have been chromosomally mapped. Four genes do not belong to any of the known homeobox gene clusters but instead map to new locations on chromosome 1 (single gene) and chromosome 5 (three genes). Sequence comparisons indicate that two of these are very closely related and represent a distinct new category of homeobox genes. The remaining four mapped genes reside in previously established murine homeobox gene clusters. Specifically, two map to the cluster HOX-1 on chromosome 6 and one each to HOX-3 and HOX-4 on chromosome 15 and 2, respectively. The ratio of newly identified homeobox genes to the previously characterized murine homeobox genes suggests that there remain several uncharacterized homeobox genes in the murine genome.
在果蝇中，已证明许多在建立体节模式和确定体节特征方面起重要作用的基因携带同源异型框序列。已克隆出30多个小鼠同源异型框基因，其中许多是基于与果蝇原型的序列同源性克隆的。在此，我们通过用一个768倍简并的寡核苷酸筛选小鼠基因组文库来报告10个新的和6个先前已知的同源异型框基因的克隆和测序，该寡核苷酸对应于同源异型域识别螺旋中最保守的8个氨基酸基序。这些新的同源异型框基因中有8个已进行染色体定位。其中4个基因不属于任何已知的同源异型框基因簇，而是分别定位于1号染色体（单个基因）和5号染色体（3个基因）上的新位置。序列比较表明，其中两个基因密切相关，代表了一类独特的新的同源异型框基因。其余4个定位基因位于先前已确定的小鼠同源异型框基因簇中。具体而言，两个定位于6号染色体上的HOX-1基因簇，另外两个分别定位于15号和2号染色体上的HOX-3和HOX-4基因簇。新鉴定的同源异型框基因与先前已鉴定的小鼠同源异型框基因的比例表明，小鼠基因组中仍有几个未鉴定的同源异型框基因。
